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bacteria:t3e:xopba

The Type III Effector XopBA from //Xanthomonas//

Author: Ralf Koebnik
Internal reviewer:
Expert reviewer: WANTED!

Class: XopBA
Family: XopBA
Prototype: XopBA (Xanthomonas euvesicatoria pv. euvesicatoria; strain 66b)
RefSeq ID: WP_227478587.1 (500 aa)
3D structure: Unknown

Biological function

How discovered?

XopBA was discovered by homology to HopW/HopPmaA in Pseudomonas syringae (70% aa sequence identity) and EspW in Escherichia coli (30% aa sequence identity) (Stavrinides & Guttman, 2004; Sandu et al., 2017).

(Experimental) evidence for being a T3E

No evidence.

Regulation

Unknown.

Phenotypes

Ectopic expression of the distant homolog in E. coli, EspW, results in formation of unique membrane protrusions (Sandu et al., 2017)

Localization

The distant homolog in E. coli, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu et al., 2017).

Enzymatic function

The distant homolog in E. coli, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu et al., 2017).

Interaction partners

The distant homolog in E. coli, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu et al., 2017).

Conservation

In xanthomonads

Yes (e.g., X. euvesicatoria, X. citri, X. transclucens, X. vasicola).

In other plant pathogens/symbionts

Yes (Acidovorax spp., Erwinia spp., Pseudomonas syringae).

References

Sandu P, Crepin VF, Drechsler H, McAinsh AD, Frankel G, Berger CN (2017). The enterohemorrhagic Escherichia coli effector EspW triggers actin remodeling in a Rac1-dependent manner. Infect. Immun. 85: e00244-17. doi: 10.1128/IAI.00244-17

Stavrinides J, Guttman DS (2004). Nucleotide sequence and evolution of the five-plasmid complement of the phytopathogen Pseudomonas syringae pv. maculicola ES4326. J. Bacteriol. 186: 5101-5115. doi: 10.1128/JB.186.15.5101-5115.2004

bacteria/t3e/xopba.txt · Last modified: 2024/08/06 15:39 by rkoebnik