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Molecular Diagnosis and Diversity for Regulated Xanthomonas
Bacterial virulence factors
This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)
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Author: John Doe
Reviewer: Jane Doe
Class: XopBG
Family: XopBG
Prototype: BER92_22150 (Xanthomonas fragariae; strain Fap21)
RefSeq ID:
3D structure:
XopBG was discovered using a machine-learning approach for the prediction of T3Es (Wagner et al., 2022, 2023).
To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the Xanthomonas euvesicatoria T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs262–574 lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the Bs2 resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs262–574 elicited HR response in the pepper line ECW20R, which encodes a functional Bs2 resistance gene.
Wagner N, Avram O, Gold-Binshtok D, Zerah B, Teper D, Pupko T (2022). Effectidor: an automated machine-learning-based web server for the prediction of type-III secretion system effectors. Bioinformatics 38: 2341-2343. DOI: 10.1093/bioinformatics/btac087
Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of Xanthomonas hortorum pv. pelargonii strain 305 and novel type III effectors identified in Xanthomonas. Front. Plant Sci. 14: 1155341. DOI: 10.3389/fpls.2023.1155341
