EuroXanth DokuWiki

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
====== The Type III Effector XopBG from //Xanthomonas// ======

Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]

Class: XopBG\\
Family: XopBG\\
Prototype: BER92_RS22150 (//Xanthomonas fragariae//; strain Fap21)\\
RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_145953988.1|WP_145953988.1]] (158 aa)\\
3D structure: Unknown

===== Biological function =====

=== How discovered? ===

XopBG was discovered using a machine-learning approach for the prediction of T3Es (Wagner //et al.//, 2022, 2023).
=== (Experimental) evidence for being a T3E ===

To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
=== Regulation ===

The //xopBG// gene contains a perfect PIP box in its promoter region and is likely co-regulated with the T3SS under control of HrpG and HrpX (Wagner //et al.//, 2023).
=== Phenotypes ===

Unknown.

=== Localization ===

Unknown.

=== Enzymatic function ===

Unknown.

=== Interaction partners ===

Unknown.

===== Conservation =====

=== In xanthomonads ===

Unique to //X. fragariae// (Wagner //et al.//, 2023).
=== In other plant pathogens/symbionts ===

//Xylophilus ampelinus//.

===== References =====

Wagner N, Avram O, Gold-Binshtok D, Zerah B, Teper D, Pupko T (2022). Effectidor: an automated machine-learning-based web server for the prediction of type-III secretion system effectors. Bioinformatics 38: 2341-2343. DOI: [[https://doi.org/10.1093/bioinformatics/btac087|10.1093/bioinformatics/btac087]]

Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of //Xanthomonas hortorum// pv. //pelargonii// strain 305 and novel type III effectors identified in //Xanthomonas//. Front. Plant Sci. 14: 1155341. DOI: [[https://doi.org/10.3389/fpls.2023.1155341|10.3389/fpls.2023.1155341]]
EuroXanth DokuWiki

User Tools

Site Tools

Page Tools
