EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopam

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
bacteria:t3e:xopam [2023/09/14 09:22] – [Biological function] rkoebnikbacteria:t3e:xopam [2025/02/24 10:31] (current) – jensboch
Line 1: Line 1:
-====== XopAM ======+====== The Type III Effector XopAM from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ +Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Internal reviewer:\\ +
-Expert reviewer:+
  
 Class: XopAM\\ Class: XopAM\\
Line 16: Line 14:
 === How discovered? === === How discovered? ===
  
 +The //xopAM// gene in //X. campstris// pv. //campestris// strain 8004 (Xcc8004) was identified as a candidate T3E due to the presence of a PIP box (TTCGB-N<sub>15</sub>-TTCGB) within the upstream sequence of its putative translational start codon (Jiang //et al.//, 2009).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 +Using the HR-inducing domain AvrBs1<sub>59-445</sub> as a type-3 translocation reporter, the N-terminal region of XopAM<sub>Xcc8004</sub> was found to act as a type 3 secretion signal, since the translational reporter fusion triggered a //Bs1//-specific hypersensitive response in a //hrpF//- and //hpaB//-dependent manner (Jiang //et al.//, 2009).
 === Regulation === === Regulation ===
  
 +Using a promoterless beta-glucuronidase (//gus//) gene as a transcriptional reporter, //xopAM// <sub>Xcc8004</sub> was found to be under control of HrpG and HrpX (Jiang //et al.//, 2009).
 === Phenotypes === === Phenotypes ===
  
Line 34: Line 35:
  
 Binding of XopAM<sub>Xcc</sub> to XOPAM-ACTIVATED RESISTANCE 1 (AMAR1) induced an intense hypersensitive response that restricted //Xcc// proliferation (Xie //et al.//, 2023). Binding of XopAM<sub>Xcc</sub> to XOPAM-ACTIVATED RESISTANCE 1 (AMAR1) induced an intense hypersensitive response that restricted //Xcc// proliferation (Xie //et al.//, 2023).
- 
 ===== Conservation ===== ===== Conservation =====
  
Line 52: Line 52:
 Mukaihara T, Tamura N, Murata Y, Iwabuchi M (2004). Genetic screening of Hrp type III-related pathogenicity genes controlled by the HrpB transcriptional activator in //Ralstonia solanacearum//. Mol. Microbiol. 54: 863-875. DOI: [[https://doi.org/10.1111/j.1365-2958.2004.04328.x|10.1111/j.1365-2958.2004.04328.x]] Mukaihara T, Tamura N, Murata Y, Iwabuchi M (2004). Genetic screening of Hrp type III-related pathogenicity genes controlled by the HrpB transcriptional activator in //Ralstonia solanacearum//. Mol. Microbiol. 54: 863-875. DOI: [[https://doi.org/10.1111/j.1365-2958.2004.04328.x|10.1111/j.1365-2958.2004.04328.x]]
  
-Xie Q, Wei B, Zhan Z, He Q, Wu K, Chen Y, Liu S, He C, Niu X, Li C, Tang C, Tao J (2023). Arabidopsis membrane protein AMAR1 interaction with type III effector XopAM triggers a hypersensitive response. Plant Physiol., in press. DOI: [[https://doi.org/10.1093/plphys/kiad478|10.1093/plphys/kiad478]]+Xie Q, Wei B, Zhan Z, He Q, Wu K, Chen Y, Liu S, He C, Niu X, Li C, Tang C, Tao J (2023). Arabidopsis membrane protein AMAR1 interaction with type III effector XopAM triggers a hypersensitive response. Plant Physiol. 193: 2768-2787. DOI: [[https://doi.org/10.1093/plphys/kiad478|10.1093/plphys/kiad478]]
  
bacteria/t3e/xopam.1694679721.txt.gz · Last modified: 2023/09/14 09:22 by rkoebnik

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki