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--- bacteria:t3e:xopab [2020/07/09 11:19] – [References] rkoebnik
+++ bacteria:t3e:xopab [2025/07/13 23:58] (current) – jfpothier
@@ Line 1: / Line 1: @@
-====== XopAB ======
+====== The Type III Effector XopAB from //Xanthomonas// ======
-Author: John Doe\\
+Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
-Reviewer: Jane Doe
 Class: XopAB\\
 Family: XopAB\\
-Prototype: Xop? (//Xanthomonas//; strain)\\
+Prototype: XOO3150 (//Xanthomonas oryzae// pv. //oryzae//; strain T7174)\\
-RefSeq ID:\\
+GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/BAE69905.1|BAE69905.1]] (193 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011409110.1| WP_011409110.1]] (193 aa)\\
 D structure: Unknown
@@ Line 13: / Line 13: @@
 === How discovered? ===
+XopAB was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al.//, 2009).
 === (Experimental) evidence for being a T3E ===
+TT3S-dependent translocation of XopAB has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al.//, 2009). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 === Regulation ===
@@ Line 34: / Line 38: @@
 ===== References =====
-Furutani A,Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// pv. //oryzae.// Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/MPMI-22-1-0096|10.1094/MPMI-22-1-0096]]
+Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// pv. //oryzae.// Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/MPMI-22-1-0096|10.1094/MPMI-22-1-0096]]
+Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]
+===== Acknowledgements =====
+This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).

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