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bacteria:t3e:xopab

The Type III Effector XopAB from //Xanthomonas//

Author: Ralf Koebnik

Class: XopAB
Family: XopAB
Prototype: XOO3150 (Xanthomonas oryzae pv. oryzae; strain T7174)
GenBank ID: BAE69905.1 (193 aa)
RefSeq ID: WP_011409110.1 (193 aa)
3D structure: Unknown

Biological function

How discovered?

XopAB was discovered via genome-wide screening for effector candidates in X. oryzae pv. oryzae (Xoo) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of P. syringae strains and X. campestris pv. vesicatoria, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani et al., 2009).

(Experimental) evidence for being a T3E

TT3S-dependent translocation of XopAB has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani et al., 2009). XopRXoo was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao et al., 2013).

Regulation

Phenotypes

Localization

Enzymatic function

Interaction partners

Conservation

In xanthomonads

In other plant pathogens/symbionts

References

Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in Xanthomonas oryzae pv. oryzae. Mol. Plant Microbe Interact. 22: 96-106. DOI: 10.1094/MPMI-22-1-0096

Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in Xanthomonas oryzae pv. oryzae Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: 10.1007/s11274-012-1229-5

bacteria/t3e/xopab.txt · Last modified: 2025/02/24 11:54 by rkoebnik