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plant:populus [2025/07/05 14:43] – jfpothier | plant:populus [2025/08/06 22:13] (current) – jfpothier | ||
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* PCR analysis of genomic DNA with specific primers to verify that the ubi7-D4E1-nos expression cassette is present in the plant genome of transgenic poplar clones (Mentag //et al.//, 2003); | * PCR analysis of genomic DNA with specific primers to verify that the ubi7-D4E1-nos expression cassette is present in the plant genome of transgenic poplar clones (Mentag //et al.//, 2003); | ||
- | * Expression of // | + | * Expression of //D4E1// tested by RT-PCR and RNA dot-blot analysis (Mentag //et al.//, 2003). |
=== Brief description === | === Brief description === | ||
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==== Pathogen elicitors ==== | ==== Pathogen elicitors ==== | ||
- | * Crude elicitors from //X. campestris// | + | * Crude elicitors from //X. campestris// |
- | * Crude elicitors from //X. populi// pv. // | + | * Crude elicitors from //X. populi// pv. //populi// strain 2551 |
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==== Genes involved in defense ==== | ==== Genes involved in defense ==== | ||
- | // | + | //MAPK// genes (Hamel //et al.//, 2005; Duplessis //et al.//, 2009). |
=== Synonyms === | === Synonyms === | ||
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=== Source === | === Source === | ||
- | Hybrid poplar cell suspension cultures of //Populus trichocarpa// | + | Hybrid poplar cell suspension cultures of //Populus trichocarpa// |
=== Status (identified, | === Status (identified, | ||
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=== Molecular markers === | === Molecular markers === | ||
- | Expression of // | + | Expression of //MAPK// genes tested by in-gel kinase assay (Hamel //et al//., 2005). |
=== Brief description === | === Brief description === | ||
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=== Brief description === | === Brief description === | ||
- | Resistant species or cultivars are determined by field observations or by controlled mechanical inoculations of the pathogen into the plants. A literature synthesis of the susceptibility of main European cultivars to //X. populi// pv. // | + | Resistant species or cultivars are determined by field observations or by controlled mechanical inoculations of the pathogen into the plants. A literature synthesis of the susceptibility of main European cultivars to //X. populi// pv. //populi// was made by Pinon & Valadon in 1995. |
**Table 1: Main European resistant cultivars to //X. populi//, according to the literature synthesis of Pinon & Valadon (1995).** | **Table 1: Main European resistant cultivars to //X. populi//, according to the literature synthesis of Pinon & Valadon (1995).** |