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Molecular Diagnosis and Diversity for Regulated Xanthomonas
Bacterial virulence factors
This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)
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Author: Roland Kölliker
Internal reviewer: Massimiliano Morelli
Expert reviewer: 
cassava4.1_013474 (Sui et al., 2017).
Cassava cultivar TMS 60444 (Cohn et al., 2014; Cohn et al., 2016).
Sequenced.
NA.
Interacts with Tal20Xam668 (Cohn et al., 2014).
NA.
Cassava cultivar TMS 60444 (Cohn et al., 2016).
Identified (Cohn et al., 2016).
NA.
RNA sequencing was used to identify the full target repertoire of TAL14Xam668 in cassava, which includes over 50 genes. A subset of highly up-regulated genes was tested for activation by TAL14CIO151 from Xam strain CIO151 (Cohn et al., 2016).
PCR250; Xa21-like resistance gene.
Cassava cultivars MBRA685 and MBRA902.
Sequenced.
PCR250.
Overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136. This suggests that RXam1 might be implicated in strain-specific resistance to XamCIO136 (Diaz-Tatis et al., 2018).
NA.
Pepper.
Sequenced.
NA.
Transgenic cassava plants that functionally express Bs2 were regenerated. Our results show that overexpression of Bs2 in a highly susceptible cultivar leads to reactive oxygen species production. However, the overexpression of Bs2 neither leads to an HR in cassava nor reduces Xam growth on in vitro plants (Diaz-Tatis et al., 2019).
NA.
Cassava.
Sequenced.
NA.
Arabidopsis MeBIK1 overexpression lines OX1 demonstrated a strong resistance to Xanthomonas axonopodis pv. manihotis HN01 (Li et al., 2017b).
Cassava.
Sequenced.
NA.
MebZIP3 and MebZIP5 conferred improved disease resistance against cassava bacterial blight, with more callose depositions.
NA.
Cassava.
Sequenced.
NA.
Through overexpression in //Nicotiana benthamiana//, we found that 4 MeDELLAs conferred improved disease resistance against cassava bacterial blight (Li et al., 2018).
NA.
Cassava cultivar MCOL22.
Sequenced.
NA.
Polymorphisms between cultivars generally reflected geographic origin, but there was also an association with resistance to CBB, indicating that MEPX1 could be a potentially useful marker for this trait (Pereira et al., 2003).
NA.
Cassava F1 mapping population.
Mapped.
Various defence related candidate genes.
Based on composite interval mapping analysis, 5 strain-specific QTL for resistance to Xam explaining between 15.8 and 22.1% of phenotypic variance, were detected and localized on a high resolution SNP-based genetic map of cassava (Soto et al., 2017).
NA.
Cassava.
Sequenced.
NA.
Gene expression assays showed that the transcripts of MeRAVs were commonly regulated after Xanthomonas axonopodis pv manihotis (Xam) and MeRAVs were specifically located in plant cell nuclei. Through virus‐induced gene silencing (VIGS) in cassava, we found that MeRAV1 and MeRAV2 are essential for plant disease resistance against cassava bacterial blight, as shown by the bacterial propagation of Xam in plant leaves (Wei et al., 2018a).
NA.
Cassava.
Sequenced.
NA.
Through transient expression in Nicotiana benthamiana leaves and virus-induced gene silencing (VIGS) in cassava, we identified the essential role of MeHsf3 in plant disease resistance (Wei et al., 2018).
NA.
Cassava.
Sequenced.
NA.
Taken together, MeWRKY20 and MeATG8a/8f/8h are essential for disease resistance against bacterial blight by forming various transcriptional modules and interacting complex in cassava (Yan et al., 2017; Zeng et al., 2018) .
Cohn M, Bart RS, Shybut M et al. (2014). Xanthomonas axonopodis virulence is promoted by a transcription activator-like effector–mediated induction of a SWEET sugar transporter in Cassava. Mol. Plant-Microbe Interact. 27: 1186-1198. DOI: 10.1094/MPMI-06-14-0161-R.
Cohn M, Morbitzer R, Lahaye T, Staskawicz J (2016). Comparison of gene activation by two TAL effectors from Xanthomonas axonopodis pv. manihotis reveals candidate host susceptibility genes in cassava. Mol. Plant Pathol. (2016) 17: 875-889. DOI: 10.1111/Fmpp.12337.
Diaz-Tatis, PA, Herrera-Corzo M, Ochoa Cabezas JC et al. (2018). The overexpression of RXam1, a cassava gene coding for an RLK, confers disease resistance to Xanthomonas axonopodis pv. manihotis. Planta 247: 1031-1042. DOI: 10.1007/s00425-018-2863-4.
Diaz-Tatis PA, Ochoa JC, Garcia L et al. (2019). Interfamily transfer of Bs2 from pepper to cassava (Manihot esculenta Crantz). Tropical Plant Pathol. (2019) 44: 225-237. DOI: 10.1007/s40858-019-00279-y.
Li X, Fan S, Hu W et al. (2017a). Two Cassava Basic Leucine Zipper (bZIP) Transcription Factors (MebZIP3 and MebZIP5) Confer Disease Resistance against Cassava Bacterial Blight. Front. Plant Sci. (2017a) DOI: 10.3389/fpls.2017.02110.
Li K, Xion X, Zhu S et al. (2017b). MeBIK1, a novel cassava receptor-like cytoplasmic kinase, regulates PTI response of transgenic Arabidopsis. Funct. Plant Biol. (2017b) 45: 658-667. DOI: 10.1071/FP17192.
Li X, Liu W, Li B et al. (2018). Identification and functional analysis of cassava DELLA proteins in plant disease resistance against cassava bacterial blight. Plant Physiol. Biochem. 124: 70-76. DOI: 10.1016/j.plaphy.2017.12.022.
Pereira LF, Goodwin PH, Erickson L (2003). Cloning of a Peroxidase Gene from Cassava with Potential as a Molecular Marker for Resistance to Bacterial Blight. Brazilian Archives of Biology and Technology 46: 149-154. DOI: 10.1590/S1516-89132003000200002.
Soto Sedana JC, Mora Moreno RE, Mathew B et al. (2017). Major Novel QTL for Resistance to Cassava Bacterial Blight Identified through a Multi-Environmental Analysis. Front. Plant Sci. 8: 1169. DOI: 10.3389/fpls.2017.01169.
Wei Y, Chang Y, Zeng H et al. (2018a). RAV transcription factors are essential for disease resistance against cassava bacterial blight via activation of melatonin biosynthesis genes. J. Pineal Res. 64: e12454. DOI: 10.1111/jpi.12454.
Wei Y, Liu G, Chang Y (2018b). Heat shock transcription factor 3 regulates plant immune response through modulation of salicylic acid accumulation and signalling in cassava. Mol. Plant Pathol. 19: 2209-2220. DOI: 10.1111/Fmpp.12691.
Yan Y, Wang P, He C, Shi H (2017). MeWRKY20 and its interacting and activating autophagy-related protein 8 (MeATG8) regulate plant disease resistance in cassava. Biochem. Biophys. Res. Commun. 494: 20-26. DOI: 10.1016/j.bbrc.2017.10.091.
Zeng H, Xie Y, Liu G et al. (2018). Molecular identification of GAPDHs in cassava highlights the antagonism of MeGAPCs and MeATG8s in plant disease resistance against cassava bacterial blight. Plant Mol. Biol. 97: 201-214. DOI: 10.1007/s11103-018-0733-x.
