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--- plant:banana [2025/05/12 08:17] – rkoebnik
+++ plant:banana [2026/04/13 09:20] (current) – [Resistance gene: //MusaPUB22/23//] rkoebnik
@@ Line 1: / Line 1: @@
 ====== Plant Resistance Genes in Banana (//Musa balbisiana// and Pisang Awak cultivar) against //Xanthomonas// Infection ======
-Author: Rita Fernandes\\
+Author: [[https://sigarra.up.pt/up/en/WEB_BASE.GERA_PAGINA?p_pagina=home|Rita Fernandes]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Noemi_Casarin2|Noemi Casarin]]
@@ Line 205: / Line 205: @@
 === Status (identified, mapped, cloned, sequenced) ===
-Mapped on chromosome 8 of sweet pepper (//Capsicum annuum)//, sequenced (Dayakar //et al//., 2003; Qin //et al//., 2014).
+Mapped on chromosome 8 of sweet pepper (//Capsicum annuum//), sequenced (Dayakar //et al//., 2003; Qin //et al//., 2014).
 === Molecular markers ===
@@ Line 215: / Line 215: @@
 ----
-==== Resistance gene: //hrap// ====
+==== Resistance gene: //MusaPUB22/23// ====
 === Synonyms ===
-Extracellular ribonuclease LE-like, AF168415.1, LOC107877517.
+//NA//
 === Source ===
-Transformed into banana cultivars ‘Sukali Ndiizi’ and ‘Nakinyika’, but isolated from sweet pepper (//Capsicum annuum//) (Tripathi //et al//., 2019).
+Susceptibility genes in the BXW-susceptible 'Sukali Ndiizi' cultivar (Tripathi //et al//., 2025).
 === Status (identified, mapped, cloned, sequenced) ===
-Identified, mapped on chromosome 7 (of sweet pepper) and sequenced (Chen //et al//., 2000; Qin //et al//., 2014).
+Identified and sequenced (Tripathi //et al//., 2025).
 === Molecular markers ===
+Edited genes (Tripathi //et al//., 2025)
+=== Brief description ===
+Genes //MusaPUB22// and //MusaPUB23// were identified through prior comparative transcriptomics analysis, which revealed their upregulation in susceptible versus resistant genotypes during early infection with //Xvm//. Several gene edited lines displayed complete resistance to //Xvm// infection, while others showed partial resistance, in contrast to the full susceptibility of wild-type controls. Resistance correlated with hydrogen peroxide accumulation and activation of key defense-related genes, indicating enhanced immune responses (Tripathi //et al//., 2025).
+----
+==== Resistance gene: //  MusaVicilin  // ====
+=== Synonyms ===
 //NA//
+=== Source ===
+//MusaVicilin// gene cloned from //Musa balbisiana// (Macharia //et al//., 2026).
+=== Status (identified, mapped, cloned, sequenced) ===
+Identified and sequenced (Macharia //et al//., 2026).
+=== Molecular markers ===
+Transgenic lines overexpressing the //MusaVicilin// gene under the control of the constitutive CaMV 35S promoter (Macharia //et al//., 2026)
 === Brief description ===
-Extracellular ribonuclease LE-like, encodes for hypersensitive response assisting protein, ribonuclease T2 activity, RNA binding. Role in intensifying the hypersensitive response mediated by harpinPSS (harpin derived from //Pseudomonas syringae// pv.// syringae//) by dissociating multimeric forms of the hairpin into dimers and monomers that trigger stronger hypersensitive cell death necrosis (Tripathi //et al.//, 2014).
+Comparative transcriptomic analyses revealed a five-fold upregulation of //MusaVicilin// gene in //M. balbisiana// (BB genome) compared to the BXW- susceptible 'Pisang Awak' at early infection stage with the pathogen. //MusaVicilin// overexpression showed moderate correlation with disease resistance, with transgenic line (S2) demonstrating complete resistance under greenhouse conditions. These results suggest that the overexpression of //MusaVicilin// can confer enhanced resistance to BXW and highlight its potential as a candidate gene for genetic engineering resistance to BXW in susceptible cultivars. responses (Macharia //et al//., 2026).
 ----
@@ Line 245: / Line 264: @@
 Hubert O, Piral G, Galas C, Baurens F-C, Mbéguié-A-Mbéguié D (2014). Changes in ethylene signaling and MADS box gene expression are associated with banana finger drop. Plant Sci. 223: 99-108. DOI: [[https://doi.org/10.1016/j.plantsci.2014.03.008|10.1016/j.plantsci.2014.03.008]]
+Macharia SW, Tripathi JN, Ntui VO, Kariuki SM, Tripathi L (2026). Overexpression of //MusaVicilin// gene for disease resistance in banana. Front. Plant Sci. 17: 1737976. DOI: [[https://doi.org/10.3389/fpls.2026.1737976|10.3389/fpls.2026.1737976]]
 Medina-Suárez R, Manning K, Fletcher J, Aked J, Bird CR, Seymour GB (1997). Gene expression in the pulp of ripening bananas (two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of //in vitro// translation products and cDNA cloning of 25 different ripening-related mRNAs). Plant Physiol. 115: 453-461. DOI: [[https://doi.org/10.1104/pp.115.2.453|10.1104/pp.115.2.453]]
@@ Line 255: / Line 276: @@
 Tripathi L, Tripathi JN, Shah T, Muiruri KS, Katari M (2019). Molecular basis of disease resistance in banana progenitor //Musa balbisiana// against //Xanthomonas campestris// pv. //musacearum//. Sci. Rep. 9: 7007. DOI: [[https://doi.org/10.1038/s41598-019-43421-1|10.1038/s41598-019-43421-1]]
+Tripathi L, Ntui VO, Muiruri S, Shah T, Tripathi JN (2025). Loss of function of //MusaPUB// genes in banana can provide enhanced resistance to bacterial wilt disease. Commun. Biol. 8: 708. DOI: [[https://doi.org/10.1038/s42003-025-08093-w|10.1038/s42003-025-08093-w]]
 ----

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