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diagnostics:dna_protocols

Molecular Methods in Diagnosis and Detection of Regulated Xanthomonads

This data is based on the review by Catara et al. (2021), focused on the molecular methods for diagnosis, detection, and studies on the diversity of plant pathogenic Xanthomonas, concentrating especially on regulated pathogens in the European Union published as a collective effort of the Working Group 1 'Diagnostics & Diversity–Population Structure' from the EuroXanth COST Action CA16107.

Main protocols described for regulated xanthomonads based on amplification of specific target DNAs.

Disease Bacteria Conventional PCR qPCR1 IA2 Methods
Onion bacterial blight X. euvesicatoria pv. alli Robène-Soustrade (2010) Robène (2015) NA3
Citrus bacterial canker X. citri pv. citri
X. aurantifolii pathotypes B and C
Hartung (1996),
Cubero (2002),
Coletta-Filho (2006),
Suk Park (2006),
Kositcharoenkul (2011),
Fonseca (2019)
Mavrodieva (2004),
Cubero (2005),
Robène (2020)
Rigano (2010),
Webster (2022),
Sidireddi (2024)
Bacterial spot of stone fruits, walnut blight, hazelnut blight X. arboricola pvs. pruni, corylina and juglandis Park (2010),
Fernandes (2017),
Jouen (2019),
Webber (2020),
Kałużna (2023)
Palacio-Bielsa (2011),
Garita-Cambronero (2017),
Martins (2019),
Kałużna (2023),
Panth (2024),
Sabuquillo (2024)
Bühlmann (2013),
Li (2021),
Kałużna (2023)
Bacterial leaf blight and bacterial leaf streak of rice X. oryzae pvs. oryzae and oryzicola Sakthivel (2001),
Kang (2008),
Lang (2010),
Cho (2011),
Shen (2012),
Singhal (2015),
Cui (2016)
Cho (2011),
Kang (2012),
Koroleva (2022)
Lang (2014),
Zhu (2022),
Buddhachat (2024)
Bacterial spot of pepper and tomato X. euvesicatoria pvs. euvesicatoria and perforans,
X. hortorum pv. gardneri,
X. vesicatoria
Moretti (2009),
Araújo (2011),
Araújo (2013),
Beran (2013),
Koenraadt (2019),
Pečenka (2020),
Siddique (2023),
Utami (2023)
Strayer (2016),
Pečenka (2020),
Siddique (2023),
Utami (2023)
Larrea-Sarmiento (2018),
Strayer-Scherer (2019),
Stehlíková (2020),
Beran (2023),
Siddique (2023)
Shymanovich (2024)
Bacterial angular leaf spot of strawberry X. fragariae Hartung (1997),
Cruz (1999),
Stöger (2004),
Moltmann (2005)
Turechek (2008),
Vandroemme (2008),
Cubero (2009)
Wang (2016), Gétaz (2017),
Wang (2020),
Immanuel (2020)
Bacterial blight of anthurium and other aroids X. phaseoli pv. dieffenbachiae Khoodoo (2005),
Robène-Soustrade (2006),
Chabirand (2014)
Jouen (2019) Jun-Hai (2015)
Bacterial leaf spot of poinsettia X. axonopodis pv. poinsetticola Back (2015) NA NA
Bacterial leaf streak and black chaff of cereals X. translucens pv. translucens Maes (1995),
Roman-Reyna (2023),
Hong (2023)
Sarkes (2022),
Tambong (2023),
Fu (2023),
Tambong (2024)
Langlois (2017),
Sarkes (2022)
Common blight of bean X. phaseoli pv. phaseoli,
X. citri pv. fuscans
Audy (1996) NA De Paiva (2020)

1 qPCR: quantitative real-time PCR; 2 IA: isothermal amplification: 3 NA: not applicable.

References

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Fu H, Fleitas MC, Sarkes A, Wang L, Yang Y, Zahr K, Harding MW, Feindel D, Kutcher R, Feng J (2024). Detection and differentiation of Xanthomonas translucens pathovars translucens and undulosa from wheat and barley by duplex quantitative PCR. Plant Dis. 108: 270-277. DOI: 10.1094/PDIS-05-23-0887-SR

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Moretti C, Amatulli MT, Buonaurio R (2009). PCR-based assay for the detection of Xanthomonas euvesicatoria causing pepper and tomato bacterial spot. Lett. Appl. Microbiol. 49: 466-471. DOI: 10.1111/j.1472-765X.2009.02690.x

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Robène I, Perret M, Jouen E, Escalon A, Maillot MV, Chabirand A, Moreau A, Laurent A, Chiroleu F, Pruvost O (2015). Development and validation of a real-time quantitative PCR assay to detect Xanthomonas axonopodis pv. allii from onion seed. J. Microbiol. Methods. 114: 78-86. DOI: 10.1016/j.mimet.2015.04.017

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Strayer-Scherer A, Jones JB, Paret ML (2019). Recombinase polymerase amplification assay for field detection of tomato bacterial spot pathogens. Phytopathology 109: 690-700. DOI: 10.1094/PHYTO-03-18-0101-R

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Tambong J, Xu R, Fleitas M, Wang L, Hubbard K, Kutcher HR (2023). Phylogenomic insights on the Xanthomonas translucens complex, and development of a TaqMan real-time assay for specific detection of pv. translucens on barley. Phytopathology 113: 2091-2102. DOI: 10.1094/PHYTO-01-23-0022-SA

Tambong JT, Xu R, Fleitas MC, Wang L, Akuma M, Chi SI, Kutcher HR (2024). TaqMan real-time PCR assay for specific detection and differentiation of Xanthomonas translucens pv. undulosa from other pathovars targeting a recombination mediator gene, recF. Plant Dis. 108: 1869-1878. DOI: 10.1094/PDIS-09-23-1827-RE

Turechek WW, Hartung JS, McCallister J (2008). Development and optimization of a real-time detection assay for Xanthomonas fragariae in strawberry crown tissue with receiver operating characteristic curve analysis. Phytopathology 98: 359-368. DOI: 10.1094/PHYTO-98-3-0359

Utami D, Meale SJ, Young AJ (2024). Bacterial leaf spot susceptibility screening of chili pepper cultivars using qPCR determination of Xanthomonas euvesicatoria pv. euvesicatoria titers. Phytopathology 114: 681-689. DOI: 10.1094/PHYTO-12-22-0479-R

Vandroemme J, Baeyen S, Van Vaerenbergh J, De Vos, Maes M (2008). Sensitive real-time PCR detection of Xanthomonas fragariae in strawberry plants. Plant Pathol. 57: 438-444. DOI: 10.1111/j.1365-3059.2007.01813.x

Wang H, Turechek WW (2016). A loop-mediated isothermal amplification assay and sample preparation procedure for sensitive detection of Xanthomonas fragariae in strawberry. PLoS ONE 11: e0147122. DOI: 10.1371/journal.pone.0147122

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Webber JB, Putnam M, Serdani M, Pscheidt JW, Wiman NG, Stockwell VO (2020). Characterization of isolates of Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight, from Oregon hazelnut orchards. J. Plant Pathol. 102: 799-812. DOI: 10.1007/s42161-020-00505-6

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diagnostics/dna_protocols.txt · Last modified: 2024/11/19 14:15 by rkoebnik