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Molecular Diagnosis and Diversity for Regulated Xanthomonas
Bacterial virulence factors
This DokuWiki is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology)
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Author: Ralf Koebnik
Internal reviewer:
Expert reviewer:
Class: XopAN
Family: XopAN
Prototype: XopAN (Xanthomonas campestris pv. campestris)
3D structure: Unknown
XopAN was originally reserved for a type 3 effector from X. campestris pv. campestris, which turned out to be related to XC_3176, a protein that was identified as a type 3 effector and nominated XopAY (see below; Yang et al., 2015). The N-terminal portion of XC_3176 is similar to XCV1197 and the C-terminal portion is similar to XCV1198. XCV1197 was shown to contain a type 3 secretion signal and was called XopAV, now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2. For more information, see also XopAY.
The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene Bs1 in a hrcV-dependent manner (Yang et al., 2015).
A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains ΔhrpX and ΔhrpG than that in the wild type X. campestris pv. campestris strain (Yang et al., 2015).
XC_3176 was found to be required for the full virulence of X. campestris pv. campestris strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang et al., 2015).
Unknown
Unknown
Unknown
Yes (e.g. X. campestris, X. hortorum, X. arboricola, X. fragariae)
No
Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel Xanthomonas euvesicatoria type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: 10.1111/mpp.12288
Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in Xanthomonas campestris pv. campestris. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: 26939454. Article in Chinese.
