Molecular Methods in Diagnosis and Detection of Regulated Xanthomonads

This data is based on the review by Catara et al. (2021), focused on the molecular methods for diagnosis, detection, and studies on the diversity of plant pathogenic Xanthomonas, concentrating especially on regulated pathogens in the European Union published as a collective effort of the Working Group 1 'Diagnostics & Diversity–Population Structure' from the EuroXanth COST Action CA16107.

Main protocols described for regulated xanthomonads based on amplification of specific target DNAs.

Disease Bacteria Conventional PCR qPCR1 IA2 Methods
Onion bacterial blight X. euvesicatoria pv. alli Robène-Soustrade (2010) Robène (2015) NA3
Citrus bacterial canker X. citri pv. citri
X. aurantifolii pathotypes B and C
Hartung (1996),
Cubero (2002),
Coletta-Filho (2006),
Suk Park (2006),
Kositcharoenkul (2011),
Fonseca (2019)
Mavrodieva (2004),
Cubero (2005),
Robène (2020)
Rigano (2010),
Webster (2022),
Sidireddi (2024)
Bacterial spot of stone fruits, walnut blight, hazelnut blight X. arboricola pvs. pruni, corylina and juglandis Park (2010),
Fernandes (2017),
Jouen (2019),
Webber (2020),
Kałużna (2023)
Palacio-Bielsa (2011),
Garita-Cambronero (2017),
Martins (2019),
Kałużna (2023),
Panth (2024),
Sabuquillo (2024)
Bühlmann (2013),
Li (2021),
Kałużna (2023)
Bacterial leaf blight and bacterial leaf streak of rice X. oryzae pvs. oryzae and oryzicola Sakthivel (2001),
Kang (2008),
Lang (2010),
Cho (2011),
Shen (2012),
Singhal (2015),
Cui (2016)
Cho (2011),
Kang (2012),
Koroleva (2022)
Lang (2014),
Zhu (2022),
Buddhachat (2024)
Bacterial spot of pepper and tomato X. euvesicatoria pvs. euvesicatoria and perforans,
X. hortorum pv. gardneri,
X. vesicatoria
Moretti (2009),
Araújo (2011),
Araújo (2013),
Beran (2013),
Koenraadt (2019),
Pečenka (2020),
Siddique (2023),
Utami (2023)
Strayer (2016),
Pečenka (2020),
Siddique (2023),
Utami (2023)
Larrea-Sarmiento (2018),
Strayer-Scherer (2019),
Stehlíková (2020),
Beran (2023),
Siddique (2023)
Shymanovich (2024)
Bacterial angular leaf spot of strawberry X. fragariae Hartung (1997),
Cruz (1999),
Stöger (2004),
Moltmann (2005)
Turechek (2008),
Vandroemme (2008),
Cubero (2009)
Wang (2016), Gétaz (2017),
Wang (2020),
Immanuel (2020)
Bacterial blight of anthurium and other aroids X. phaseoli pv. dieffenbachiae Khoodoo (2005),
Robène-Soustrade (2006),
Chabirand (2014)
Jouen (2019) Jun-Hai (2015)
Bacterial leaf spot of poinsettia X. axonopodis pv. poinsetticola Back (2015) NA NA
Bacterial leaf streak and black chaff of cereals X. translucens pv. translucens Maes (1995),
Roman-Reyna (2023),
Hong (2023)
Sarkes (2022),
Tambong (2023),
Fu (2023),
Tambong (2024)
Langlois (2017),
Sarkes (2022)
Common blight of bean X. phaseoli pv. phaseoli,
X. citri pv. fuscans
Audy (1996) NA De Paiva (2020)

1 qPCR: quantitative real-time PCR; 2 IA: isothermal amplification: 3 NA: not applicable.

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