Table of Contents

The Type III Effector XopAY from //Xanthomonas// (replaced by XopAV)

Author: Ralf Koebnik
Internal reviewer:
Expert reviewer:

Class: XopAY
Family: XopAY
Prototype: XC_3176 (Xanthomonas campestris pv. campestris; strain 8004)
GenBank ID: AAY50220.1 (244 aa)
3D structure: Unknown

Biological function

How discovered?

XopAY was originally assigned to XC_3176, a protein that was identified as a type 3 effector (see below; Yang et al., 2015). The N-terminal portion of XC_3176 is similar to XCV1197 and the C-terminal portion is similar to XCV1198. XCV1197 was shown to contain a type 3 secretion signal and was called XopAV, now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2.

XopAV1          ------------------------------------------------------------
XCV1197         MRREQCTKQCRRGRSCAGAACLGRWRGGIGAGCGGQRARSTRSPATLLVCLQRYVARACT
XCV1198         ------------------------------------------------------------
XC_3176         ------------------------------------------------------------
XopAV2          ------------------------------------------------------------

XopAV1          -------------------------------MARIHSGEVTMRPLALTSVTRRGARREQQ
XCV1197         ACALWQAPCRLFRVRQWFFGRMRHARSRWGTMARIHSGEVTMRPLALTSVTRRGARREQQ
XCV1198         ------------------------------------------------------------
XC_3176         ----------------------------------------------MTSVAREAVRHDQR
XopAV2          -----------------------------------------MGSFPVTSVAREAVRHDQR

XopAV1          EGATVADAGPPAPANDPHANTQLAQSLPRRPVREARLRAPGTPRGVPLVNLGRTMAHLGA
XCV1197         EGATVADAGPPAPANDPHANTQLAQSLPRRPTAISTMTCITRILL---------------
XCV1198         ------------------------------------------------------------
XC_3176         EAQAPREVGYSSQRGDPQANPQLKQDLSRAPVRAARLKAPRVPIGVHIANTGLQAARFAT
XopAV2          EAQAPREVGYSSQRGDPQANPQLKQDLSRAPVRAARLKAPRVPIGVHIANTGLQAARFAT

XopAV1          ---VGAAVWGGFAAIQAPAAAAYGYFNDDLYYKNLAVVSGYQAVGALAAFLGMGLADVLA
XCV1197         ------------------------------------------------------------
XCV1198         -------------------------------------------MGALAAFLGMGLADVLA
XC_3176         SAVAGPAEAAVLDAVHAPAAAAYDYAKGEVSWKKNAGVTLAPALAYAVVMGTLSAVQYLA
XopAV2          SAVAGPAEAAVLDAVHAPAAAAYDYAKGEVSWKKNAGVTLAPALAYAVVMGTLSAVQYLA

XopAV1          ARYVEAQSRWYEVPTKAQRASELGATEACLDQAVDMVTRLDADPPGPGMDEGERLALARD
XCV1197         ------------------------------------------------------------
XCV1198         ARYVEAQSRWYEVPTKAQRASELGATEACLDHAVDMVTIFDADHPGPAMDGGEQLVLATD
XC_3176         NRYLQAQSQ--TSVSKADRAKELGVTEACLDAAAAMVNKGEVAGPGGEMTEEEAAALAAD
XopAV2          NRYLQAQSQ--TSVSKADRAKELGVTEACLDAAAAMVNKGEVAGPGGEMTEEEAAALAAD

XopAV1          LMHLMTVERSRLPPDLWRAARGAGNDAYGLVARLLQAARARAAEEASTSSAG
XCV1197         ----------------------------------------------------
XCV1198         LLHLMTVERSRLPPELWQAASGVGDDAHRLVTQLLQAARARAADEASTSSAD
XC_3176         LQNLATADRSRLPPELWRAASGVGDDAYALVTQLLLAARARASAEASASSDG
XopAV2          LQNLATADRSRLPPELWRAASGVGDDAYALVTQLLLAARARASAEASASSDG

(Experimental) evidence for being a T3E

The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene Bs1 in a hrcV-dependent manner (Yang et al., 2015).

Regulation

A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains ΔhrpX and ΔhrpG than that in the wild type X. campestris pv. campestris strain (Yang et al., 2015).

Phenotypes

XC_3176 was found to be required for the full virulence of X. campestris pv. campestris strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang et al., 2015).

Localization

Unknown

Enzymatic function

Unknown

Interaction partners

Unknown

Conservation

In xanthomonads

Yes (e.g. X. campestris, X. hortorum, X. arboricola, X. fragariae)

In other plant pathogens/symbionts

No

References

Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel Xanthomonas euvesicatoria type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: 10.1111/mpp.12288

Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in Xanthomonas campestris pv. campestris. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: 26939454. Article in Chinese.