The Type III Effector XopAT from //Xanthomonas//

Author: Ralf Koebnik

Class: XopAT
Family: XopAT
Prototype: XCR_1464a (Xanthomonas campestris pv. raphani; strain 756C)
GenBank ID: AEL06363.1 (211 aa)
RefSeq ID: WP_148263049.1 (155 aa, probably too short)
3D structure: Unknown

xopAT was predicted as a novel type 3 effector (T3E) gene because of four criteria (Bogdanove et al., 2011):

1. The gene is encoded next to the hrp gene cluster.
2. The gene contains a plant-inducible promoter (PIP) box and a properly positioned 􏰃10 box-like sequence upstream of the coding sequence.
3. Like other T3E genes, xopAT has an unusual (low) G􏰅+C content suggestive of acquisition by horizontal gene transfer.
4. In common with other T3Es, the encoded protein contains eukaryotic motifs for myristoylation and palmitoylation.

Yes (X. arboricola, X. campestris, X. codiaei, X. cucurbitae, X. fragariae, X. hortorum)

No

Bogdanove AJ, Koebnik R, Lu H, Furutani A, Angiuoli SV, Patil PB, Van Sluys MA, Ryan RP, Meyer DF, Han SW, Aparna G, Rajaram M, Delcher AL, Phillippy AM, Puiu D, Schatz MC, Shumway M, Sommer DD, Trapnell C, Benahmed F, Dimitrov G, Madupu R, Radune D, Sullivan S, Jha G, Ishihara H, Lee SW, Pandey A, Sharma V, Sriariyanun M, Szurek B, Vera-Cruz CM, Dorman KS, Ronald PC, Verdier V, Dow JM, Sonti RV, Tsuge S, Brendel VP, Rabinowicz PD, Leach JE, White FF, Salzberg SL (2011). Two new complete genome sequences offer insight into host and tissue specificity of plant pathogenic Xanthomonas spp. J. Bacteriol. 193: 5450-5464. DOI: 10.1128/JB.05262-11