====== Plant Resistance Genes in Italian ryegrass (//Lolium multiflorum// L.) against //Xanthomonas// Infection ====== Author: [[https://www.researchgate.net/profile/Florian_Goettelmann|Florian Goettelmann]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Jaroslav_Michalko|Jaroslav Michalko]]\\ Expert reviewer: [[https://www.researchgate.net/profile/Roland_Koelliker|Roland Kölliker]] ===== Pathogen: //Xanthomonas transluscens// pv. //graminis// (//Xtg//) ===== ==== Resistance gene: major LG4 QTL ==== === Synonyms === //NA// === Source === M2289. === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === LpSSRK05A11 (SSR), P38M50_252 (AFLP). === Brief description === Explained 66.8% of resistance in one glasshouse experiment and 43.3% or 83.8% of resistance in two field experiments (Studer //et al.//, 2006). ---- ==== Resistance gene: minor LG4 QTL ==== === Synonyms === //NA// === Source === M2289. === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === P32M50_410 (AFLP). === Brief description === Explained 10.8% of resistance in one field experiment (Studer //et al.//, 2006). ---- ==== Resistance gene: minor LG1 QTL ==== === Synonyms === //NA// === Source === M2289. === Status (identified, mapped, cloned, sequenced) === Mapped on LG1. === Molecular markers === LMgSSR04-06F (SSR). === Brief description === Explained 2.9% of resistance in one field experiment (Studer //et al.//, 2006). ---- ==== Resistance gene: minor LG5 QTL ==== === Synonyms === //NA// === Source === M2289. === Status (identified, mapped, cloned, sequenced) === Mapped on LG5. === Molecular markers === E41M47_133 (AFLP). === Brief description === Explained 7.4% of resistance in one glasshouse experiment (Studer //et al.//, 2006). ---- ==== Resistance gene: minor LG6 QTL ==== === Synonyms === //NA// === Source === M2289. === Status (identified, mapped, cloned, sequenced) === Mapped on LG6. === Molecular markers === E39M50_196 (AFLP). === Brief description === Explained 7.3% of resistance in one field experiment (Studer //et al.//, 2006). ---- ==== Candidate gene: F-BstCT-MseCA-188 ==== === Synonyms === CX066043. === Source === « genotype A » (advanced breeding germplasm, Agroscope FAL Reckenholz, Switzerland). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Homology to an ERBP transcription factor in //Oryza sativa// (Rechsteiner //et al.//, 2006). ---- ==== Candidate gene: F-BstTT-MseGG-159 ==== === Synonyms === CX066053. === Source === « genotype A » (advanced breeding germplasm, Agroscope FAL Reckenholz, Switzerland). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Homology to a transforming growth factor TGF-β receptor-interacting protein from //O. sativa// (Rechsteiner //et al.//, 2006) ---- ==== Candidate gene: F-BstCG-MseGT-220 ==== === Synonyms === CX066037. === Source === « genotype A » (advanced breeding germplasm, Agroscope FAL Reckenholz, Switzerland). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Homology to an EST sequence derived from //Vitis vinifera// infected with //Xylella fastidiosa// (Rechsteiner //et al.//, 2006) ---- ==== Candidate gene: F-BstCA-MseTG-50 ==== === Synonyms === CX066030. === Source === « genotype A » (advanced breeding germplasm, Agroscope FAL Reckenholz, Switzerland). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Homology to guanine nucleotide-binding protein beta (GB1) in //Oryza sativa// (Rechsteiner //et al.//, 2006). ---- ==== Candidate gene: //r_010d_c04// ==== === Synonyms === Lsi1. === Source === LmB-01 (Syn1 progeny). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Belongs to the nodulin26-like major intrinsic protein sub-family of aquaporins (Wichmann //et al.//, 2011) . ---- ==== Candidate gene: r_010d_c02 ==== === Synonyms === //NA// === Source === LmB-01 (Syn1 progeny). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Belongs to the germin-like protein family (Wichmann //et al.//, 2011). ---- ==== Candidate gene: rg6_008b_d08 ==== === Synonyms === //NA// === Source === LmB-01 (Syn1 progeny). === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === //NA// === Brief description === Synaptobrevin-like vesicle-associated membrane protein (Wichmann //et al.//, 2011) ---- ==== Candidate gene: ve_007b_e10 ==== === Synonyms === MFS18. === Source === LmB-01 (Syn1 progeny). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Flowering protein MFS18 (Wichmann //et al.//, 2011). ---- ==== Candidate gene: gsa_007c_h09 ==== === Synonyms === MFS18. === Source === LmB-01 (Syn1 progeny). === Status (identified, mapped, cloned, sequenced) === Identified. === Molecular markers === //NA// === Brief description === Ankyrin (ANK) repeat protein (Wichmann //et al.//, 2011). ---- ==== Candidate gene: AT5G47850; CRINKLY4-related protein ==== === Synonyms === //NA// === Source === Xtg-ART (M2289 x Adret). === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === SNP markers. === Brief description === CRINKLY4 (serine/ threonine protein kinase-like protein CCR4)-related protein (Knorst //et al.//, 2019). ---- ==== Candidate gene: AT3G07960; SWEET17 ==== === Synonyms === //NA// === Source === Xtg-ART (M2289 x Adret). === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === SNP markers. === Brief description === Nodulin MtN3 family protein (SWEET17) (Knorst //et al.//, 2019). ---- ==== Candidate gene: Brassinosteroid Insensitive 1 (BRI1)-associated kinase-interacting receptor-like kinase 1 ==== === Synonyms === BAK1. === Source === Xtg-ART (M2289 x Adret). === Status (identified, mapped, cloned, sequenced) === Mapped on LG4. === Molecular markers === SNP markers. === Brief description === BAK1-interacting receptor-like kinase 1 (Knorst //et al.//, 2019) . ---- ==== Candidate gene: Pik-2-like disease resistance protein (evm.TU.chr4.21718) ==== === Synonyms === Pik2-like === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ==== Candidate gene: Cystein-rich kinase 6 (evm.TU.chr4.21727) ==== === Synonyms === CRK 6 === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ==== Candidate gene: Cystein-rich kinase 8 (evm.TU.chr4.21737/8) ==== === Synonyms === CRK 8 === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ==== Candidate gene: Serine protease inhibitor (evm.TU.chr4.21747-50) ==== === Synonyms === SERPIN === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ==== Candidate gene: Resistance gene analog 4-like disease resistance protein (evm.TU.chr4.21754) ==== === Synonyms === RGA5-like === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ==== Candidate gene: Resistance gene analog 5-like disease resistance protein (XLOC_055978/76) ==== === Synonyms === RGA5-like === Source === F2 mapping population derived from XtgArt03 (Studer et al. 2006). === Status (identified, mapped, cloned, sequenced) === Identified and sequenced on LG 4 === Molecular markers === SNP markers / gene sequence === Brief description === High-resolution bulked segregant analysis identified a ~300kb region associated with bacterial wilt resistance in Italian ryegrass (Goettelmann et al. 2024) ---- ===== References ===== Goettelmann F, Yutang C, Knorst V, Yates S, Copetti D, Studer B, Kölliker R (2024). High resolution bulked segregant analysis enables candidate gene identification for bacterial wilt resistance in Italian ryegrass (//Lolium multiflorum// Lam.). Plant J., in press. DOI: [[https://doi.org/10.1111/tpj.16693|10.1111/tpj.16693]] Knorst V, Byrne S, Yates S, Asp T, Widmer F, Studer B, Kölliker R (2019). Pooled DNA sequencing to identify SNPs associated with a major QTL for bacterial wilt resistance in Italian ryegrass (//Lolium multiflorum// Lam.). Theor. Appl. Genet. 132: 947-958. DOI: [[https://doi.org/10.1007/s00122-018-3250-z|10.1007/s00122-018-3250-z]] Rechsteiner MP, Widmer F, Kölliker R (2006). Expression profiling of Italian ryegrass (Lolium multiflorum Lam.) during infection with the bacterial wilt inducing pathogen //Xanthomonas translucens// pv. //graminis//. Plant Breeding 125: 43-51. DOI: [[https://doi.org/10.1111/j.1439-0523.2006.01170.x|10.1111/j.1439-0523.2006.01170.x]] Studer B, Boller B, Herrmann D, Bauer E, Posselt UK, Widmer F, Kölliker R (2006). Genetic mapping reveals a single major QTL for bacterial wilt resistance in Italian ryegrass (//Lolium multiflorum// Lam.). Theor. Appl. Genet. 113: 661-671. DOI: [[https://doi.org/10.1007/s00122-006-0330-2|10.1007/s00122-006-0330-2]] Wichmann F, Asp T, Widmer F, Kölliker R (2011). Transcriptional responses of Italian ryegrass during interaction with //Xanthomonas translucens// pv. //graminis// reveal novel candidate genes for bacterial wilt resistance. Theor. Appl. Genet. 122: 567-579. DOI: [[https://doi.org/10.1007/s00122-010-1470-y|10.1007/s00122-010-1470-y]] ---- ===== Further reading ===== Sapkota S, Mergoum M, Liu Z (2020). The translucens group of //Xanthomonas translucens//: complicated and important pathogens causing bacterial leaf streak on cereals. Mol. Plant Pathol. 21: 291‐302. DOI: [[https://doi.org/10.1111/mpp.12909|doi:10.1111/mpp.12909]] ---- ===== Acknowledgements ===== This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).