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bacteria:t3e:xopy [2020/06/10 11:34] – [Biological function] rkoebnikbacteria:t3e:xopy [2025/07/04 23:48] (current) – jfpothier
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-====== XopY ======+====== The Type III Effector XopY from //Xanthomonas// ======
  
-Author: Irena Mačionienė\\ +Author: [[https://www.researchgate.net/profile/Irena_Macioniene|Irena Mačionienė]]\\ 
-Internal reviewer: Lucas Morinière\\ +Internal reviewer: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]]\\
-Expert reviewer: FIXME+
  
 Class: XopY\\ Class: XopY\\
 Family: XopY\\ Family: XopY\\
-Prototype: XopY, aka Xoo1488 (//Xanthomonas oryzae// pv. //oryzicola// BLS256)\\ +Prototype: XOO1488 (//Xanthomonas oryzae// pv. //oryzae//; strain T7174)\\ 
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/AEQ97580.1|AEQ97580]] (276 aa) +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/BAE68243.1|BAE68243.1]] (281 aa)\\ 
 +RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011408080.1|WP_011408080.1]] (281 aa)\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopY was discovered by screening the genome of //X. oryzae //pv. //oryzae// MAFF311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010). +XopY was discovered by screening the genome of //X. oryzae// pv. //oryzae// MAFF 311018 for proteins displaying a N-terminal amino acid pattern associated with T3S substrates in //Pseudomonas syringae// (Furutani //et al.//, 2009). It has been primarily referred to as XOO1488, and then XopY (Song & Yang, 2010).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-//X. oryzae //pv. //oryzae //transformants containing a plasmidic fusion of XopY (= XOO1488) with the Cya translocation reporter system were inoculated in tomato leaves. An increase of cAMP in the inflitrated areas was observed, thus revealing translocation of the fused protein into plant cells (Furutani //et al.//, 2009). +//X. oryzae// pv. //oryzae// transformants containing a plasmidic fusion of XopY (= XOO1488) with the Cya translocation reporter system were inoculated in tomato leaves. An increase of cAMP in the inflitrated areas was observed, thus revealing translocation of the fused protein into plant cells (Furutani //et al.//, 2009).
 === Regulation === === Regulation ===
  
-XopY from //X. oryzae //pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Yamaguchi //et al//., 2013a). Also, it was shown to be regulated by HrpX (Furutani //et al.//, 2009).+XopY from //X. oryzae// pv. //oryzae// posseses a PIP and ‐10 box in the promoter region (TTCGB‐N<sub>15</sub>‐TTCGB‐N<sub>30–32</sub>‐YANNNT) (Yamaguchi //et al//., 2013a). Also, it was shown to be regulated by HrpX (Furutani //et al.//, 2009). 
 + 
 +qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016).
  
 === Phenotypes === === Phenotypes ===
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 XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, XopY is phosphorylated by OsRLCK185, suggesting that modification of XopY in host cell may affect their virulent activity (Yamaguchi //et al//., 2013b). XopY was demonstrated to target OsRLCK185. Expression of XopY in rice cells compromises OsRLCK185-mediated immune responses, which is consistent with the fact that Xoo1488 inhibits trans-phosphorylation of the activation domain of OsRLCK185 by OsCERK1. Interestingly, XopY is phosphorylated by OsRLCK185, suggesting that modification of XopY in host cell may affect their virulent activity (Yamaguchi //et al//., 2013b).
- 
 ===== Conservation ===== ===== Conservation =====
  
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 Yes (//e.g.//, //X. oryzae//, //X. translucens, X. vasicola//). Yes (//e.g.//, //X. oryzae//, //X. translucens, X. vasicola//).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 ===== References ===== ===== References =====
  
-Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in// Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/MPMI-22-1-0096|10.1094/MPMI-22-1-0096]]+Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/MPMI-22-1-0096|10.1094/MPMI-22-1-0096]]
  
 Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae// pv. //oryzicola// suppresses rice immunity and promotes disease development. Mol. Plant Microbe Interact. 28: 869-880. DOI: [[https://doi.org/10.1094/MPMI-10-14-0314-R|10.1094/MPMI-10-14-0314-R]] Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in //Xanthomonas oryzae// pv. //oryzicola// suppresses rice immunity and promotes disease development. Mol. Plant Microbe Interact. 28: 869-880. DOI: [[https://doi.org/10.1094/MPMI-10-14-0314-R|10.1094/MPMI-10-14-0314-R]]
  
-Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ<sub>PXO99</sub> in //Xanthomonas oryzae //pv. //oryzae//. Mol. Plant Microbe Interact. 23: 893-902. DOI: [[https://doi.org/10.1094/MPMI-23-7-0893|10.1094/MPMI-23-7-0893]]+Liu Y, Long J, Shen D, Song C (2016). //Xanthomonas oryzae// pv. //oryzae// requires H-NS-family protein XrvC to regulate virulence during rice infection. FEMS Microbiol. Lett. 363: fnw067. DOI: [[https://doi.org/10.1093/femsle/fnw067|10.1093/femsle/fnw067]]
  
-Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013a). Suppression of rice immunity by //Xanthomonas oryzae //type III effector Xoo2875. Biosci. Biotechnol. Biochem. 77: 796-801. DOI: [[https://doi.org/10.1271/bbb.120929|10.1271/bbb.120929]]+Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ<sub>PXO99</sub> in //Xanthomonas oryzae// pv. //oryzae//. Mol. Plant Microbe Interact. 23: 893-902. DOI: [[https://doi.org/10.1094/MPMI-23-7-0893|10.1094/MPMI-23-7-0893]] 
 + 
 +Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013a). Suppression of rice immunity by //Xanthomonas oryzae// type III effector Xoo2875. Biosci. Biotechnol. Biochem. 77: 796-801. DOI: [[https://doi.org/10.1271/bbb.120929|10.1271/bbb.120929]]
  
 Yamaguchi K, Yamada K, Ishikawa K, Yoshimura S, Hayashi N, Uchihashi K, Ishihama N, Kishi-Kaboshi M, Takahashi A, Tsuge S, Ochiai H, Tada Y, Shimamoto K, Yoshioka H, Kawasaki T (2013b). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347-357. DOI: [[https://doi.org/10.1016/j.chom.2013.02.007|10.1016/j.chom.2013.02.007]] Yamaguchi K, Yamada K, Ishikawa K, Yoshimura S, Hayashi N, Uchihashi K, Ishihama N, Kishi-Kaboshi M, Takahashi A, Tsuge S, Ochiai H, Tada Y, Shimamoto K, Yoshioka H, Kawasaki T (2013b). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347-357. DOI: [[https://doi.org/10.1016/j.chom.2013.02.007|10.1016/j.chom.2013.02.007]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopy.1591785279.txt.gz · Last modified: 2023/01/09 10:20 (external edit)

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