EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopy

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
bacteria:t3e:xopy [2025/02/13 12:59] – jfpothierbacteria:t3e:xopy [2025/07/04 23:48] (current) – jfpothier
Line 2: Line 2:
  
 Author: [[https://www.researchgate.net/profile/Irena_Macioniene|Irena Mačionienė]]\\ Author: [[https://www.researchgate.net/profile/Irena_Macioniene|Irena Mačionienė]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]]+Internal reviewer: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]]\\
  
 Class: XopY\\ Class: XopY\\
Line 24: Line 24:
  
 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopY//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016).
 +
 === Phenotypes === === Phenotypes ===
  
 Transgenic rice plants expressing XOO1488 (Xoo1488-OX) were generated and inoculated with the T3SS-deficient //hrpX// mutant of //Xoo//, which is incapable of type III effector delivery. The //Xoo hrpX// mutant did not cause lesions in wild-type plant, presumably because of strong induction of PTI. In contrast, Xoo1488-OX plants had severe disease symptoms following infection with the //Xoo hrpX// mutant. Bacterial populations of the //Xoo hrpX// mutant in Xoo1488-OX leaves were higher than in wild-type plants. The growth of wild-type isolate //Xoo// MAFF311018 in Xoo1488-OX plants also increased significantly over its growth in wild-type plants. Thus, it is likely that XopY inhibits PTI induced by infection of //Xoo hrpX// mutant. However, it was noted that XopY knockout strain did not exhibit any defect in virulence (Yamaguchi //et al//., 2013a). XopY was also shown to inhibit chitin-induced expression of defense-related genes (Yamaguchi //et al.//, 2013b). XopY of //X. oryzae// pv. //oryzicola// was also shown to trigger HR in non-host //Nicothiana benthamiana// plants (Li //et al.//, 2015). Transgenic rice plants expressing XOO1488 (Xoo1488-OX) were generated and inoculated with the T3SS-deficient //hrpX// mutant of //Xoo//, which is incapable of type III effector delivery. The //Xoo hrpX// mutant did not cause lesions in wild-type plant, presumably because of strong induction of PTI. In contrast, Xoo1488-OX plants had severe disease symptoms following infection with the //Xoo hrpX// mutant. Bacterial populations of the //Xoo hrpX// mutant in Xoo1488-OX leaves were higher than in wild-type plants. The growth of wild-type isolate //Xoo// MAFF311018 in Xoo1488-OX plants also increased significantly over its growth in wild-type plants. Thus, it is likely that XopY inhibits PTI induced by infection of //Xoo hrpX// mutant. However, it was noted that XopY knockout strain did not exhibit any defect in virulence (Yamaguchi //et al//., 2013a). XopY was also shown to inhibit chitin-induced expression of defense-related genes (Yamaguchi //et al.//, 2013b). XopY of //X. oryzae// pv. //oryzicola// was also shown to trigger HR in non-host //Nicothiana benthamiana// plants (Li //et al.//, 2015).
 +
 === Localization === === Localization ===
  
Line 43: Line 45:
  
 Yes (//e.g.//, //X. oryzae//, //X. translucens, X. vasicola//). Yes (//e.g.//, //X. oryzae//, //X. translucens, X. vasicola//).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xopy.txt · Last modified: 2025/07/04 23:48 by jfpothier

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki