EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopx

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
bacteria:t3e:xopx [2025/07/24 22:54] – jfpothierbacteria:t3e:xopx [2025/11/29 17:52] (current) – jfpothier
Line 3: Line 3:
 Author: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]] & [[https://www.researchgate.net/profile/Sohini_Deb|Sohini Deb]]\\ Author: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]] & [[https://www.researchgate.net/profile/Sohini_Deb|Sohini Deb]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Coline-Sciallano|Coline Sciallano]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Coline-Sciallano|Coline Sciallano]]\\
-Expert reviewer: [[https://www.researchgate.net/profile/Ramesh_Sonti|Ramesh V. Sonti]]\\+Expert reviewer: [[https://www.researchgate.net/profile/Ramesh_Sonti|Ramesh V Sonti]]\\
  
 Class: XopX\\ Class: XopX\\
Line 31: Line 31:
   * During infection of rice (//Oryza sativa//) with //Xanthomonas oryzae// pv. //oryzae//, XopX was shown to be an inhibitor of rice innate immune response, as it suppresses LipA-induced callose deposition (Sinha //et al//., 2013).   * During infection of rice (//Oryza sativa//) with //Xanthomonas oryzae// pv. //oryzae//, XopX was shown to be an inhibitor of rice innate immune response, as it suppresses LipA-induced callose deposition (Sinha //et al//., 2013).
   * XopX is required for the development of //X. euvesicatoria//- induced symptoms in the bacterial spot disease of tomato (//Solanum lycopersicum//) and pepper (//Capsicum annuum//). Indeed, it promotes ethylene production, and therefore chlorosis and plant cell death during infection by //X. euvesicatoria// of susceptible tomato and in transient expression assays in tobacco. Interestingly, it also suppresses flagellin-induced production of reactive oxygen species (ROS) while promoting the accumulation of pattern-triggered immunity (PTI) gene transcripts (Stork //et al//., 2015). Eventually, the complex behavior of XopX //in planta//, which combines activation and suppression of immunity-related plant responses at the same time, allows to classify this effector with the T3Es that activates the plant ‘default to death and defense’ response (Lindeberg //et al//., 2012; Stork //et al//., 2015).   * XopX is required for the development of //X. euvesicatoria//- induced symptoms in the bacterial spot disease of tomato (//Solanum lycopersicum//) and pepper (//Capsicum annuum//). Indeed, it promotes ethylene production, and therefore chlorosis and plant cell death during infection by //X. euvesicatoria// of susceptible tomato and in transient expression assays in tobacco. Interestingly, it also suppresses flagellin-induced production of reactive oxygen species (ROS) while promoting the accumulation of pattern-triggered immunity (PTI) gene transcripts (Stork //et al//., 2015). Eventually, the complex behavior of XopX //in planta//, which combines activation and suppression of immunity-related plant responses at the same time, allows to classify this effector with the T3Es that activates the plant ‘default to death and defense’ response (Lindeberg //et al//., 2012; Stork //et al//., 2015).
-  *XopX is required for full virulence in //Xanthomonas axonopodis// pv. //manihotis//CIO151 strain. A //Xam// strain deleted for //xopX//  showed decreased ability to produce symptoms in leaves of cassava. Also, the //xopX// KO reached more than one log unit lower populations than those observed for the wild‐type strain. This underlies the importance of this effector for disease developement (Medina //et al//., 2018). +  * XopX is required for full virulence in //Xanthomonas axonopodis// pv. //manihotis// CIO151 strain. A //Xam// strain deleted for //xopX//  showed decreased ability to produce symptoms in leaves of cassava. Also, the //xopX// KO reached more than one log unit lower populations than those observed for the wild‐type strain. This underlies the importance of this effector for disease developement (Medina //et al//., 2018). 
-  * When expressed in //Pseudomonas fluorescens// 55 (//Pf//55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in //Arabidopsis// Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologuous system, XopX is not able to suppress the HR triggered by Pf 55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina //et al//., 2018). +  * When expressed in //Pseudomonas fluorescens// 55 (//Pf//55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in //Arabidopsis// Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologous system, XopX is not able to suppress the HR triggered by Pf 55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina //et al//., 2018). 
-  * When transiently expressed in //N. benthamiana// by //Agrobacterium tumefaciens//–mediated expression system, XopX from //X. oryzae// pv. //oryzicola// cause the nonhost HR at approximately 2 days (Li //et al., //2015). +  * When transiently expressed in //N. benthamiana// by //Agrobacterium tumefaciens//–mediated expression system, XopX from //X. oryzae// pv. //oryzicola// cause the nonhost HR at approximately 2 days (Li //et al//., 2015). 
-  * A ∆//xopX// mutant strain of //Xanthomonas phaseoli// pv. //manihotis// (aka //Xanthomonas axonopodis// pv. //manihotis//) showed reduced growth //in planta// and delayed spread through the vascular system of cassava (Mutka //et al.//, 2016).+  * A Δ//xopX// mutant strain of //Xanthomonas phaseoli// pv. //manihotis// (aka //Xanthomonas axonopodis// pv. //manihotis//) showed reduced growth //in planta// and delayed spread through the vascular system of cassava (Mutka //et al.//, 2016).
   * //Agrobacterium//-mediated transient expression of both //Xanthomonas oryzae// pv. //oryzae// XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).   * //Agrobacterium//-mediated transient expression of both //Xanthomonas oryzae// pv. //oryzae// XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).
   * A screen for //Xanthomonas oryzae// pv. //oryzae// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).   * A screen for //Xanthomonas oryzae// pv. //oryzae// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
-  *(ACD57163) is one among the five classes of virulence genes found to be upregulated in the //Xanthomonas oryzae// pv. //oryzae// MAI1 strain during infection. //xopX// was up-regulated at both 3 and 6 dai (Soto-Suárez //et al.//, 2010) +  * (ACD57163) is one among the five classes of virulence genes found to be upregulated in the //Xanthomonas oryzae// pv. //oryzae// MAI1 strain during infection. //xopX// was up-regulated at both 3 and 6 dai (Soto-Suárez //et al.//, 2010) 
-  *The //xopX// gene contributes to the virulence of //Xanthomonas campestris// pv. //vesicatoria// on hosts pepper and tomato. XopX targets the innate immune response, resulting in enhanced plant disease susceptibility (Metz //et al//., 2005). +  * The //xopX// gene contributes to the virulence of //Xanthomonas campestris// pv. //vesicatoria// on hosts pepper and tomato. XopX targets the innate immune response, resulting in enhanced plant disease susceptibility (Metz //et al//., 2005). 
-  *A //Xanthomonas campestris//pv. //vesicatoria xopX// mutant strain could not induce cell death response in //N. benthamiana//, and could be complemented back to cell death response on //N. benthamiana// in trans with plasmid subclones of //xopX// (Metz //et al//., 2005).+  * A //Xanthomonas campestris//pv. //vesicatoria xopX// mutant strain could not induce cell death response in //N. benthamiana//, and could be complemented back to cell death response on //N. benthamiana// in trans with plasmid subclones of //xopX// (Metz //et al//., 2005).
  
 === Localization === === Localization ===
Line 52: Line 52:
  
   * It has been suggested that XopX-triggering of plant cell death response was dependent on another cofactor delivered by the T3SS, yet still unknown (Metz //et al.//, 2005).   * It has been suggested that XopX-triggering of plant cell death response was dependent on another cofactor delivered by the T3SS, yet still unknown (Metz //et al.//, 2005).
-  *The //Xanthomonas oryzae// pv. //oryzae// XopX interacts with two of the eight rice 14-3-3 proteins, GF14d and GF14e. Mutants of XopX that are defective in 14-3-3 binding are also defective in suppression of immune responses, suggesting that interaction with 14-3-3 proteins is required for suppression of host innate immunity (Deb //et al.//, 2020). +  * The //Xanthomonas oryzae// pv. //oryzae// XopX interacts with two of the eight rice 14-3-3 proteins, GF14d and GF14e. Mutants of XopX that are defective in 14-3-3 binding are also defective in suppression of immune responses, suggesting that interaction with 14-3-3 proteins is required for suppression of host innate immunity (Deb //et al//., 2020). 
-  *Yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-IP assays indicate that the //Xanthomonas oryzae// pv. //oryzae// XopX interacts with the type III effector XopQ (Deb //et al.//, 2020).+  * Yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and co-IP assays indicate that the //Xanthomonas oryzae// pv. //oryzae// XopX interacts with the type III effector XopQ (Deb //et al.//, 2020).
  
 ===== Conservation ===== ===== Conservation =====
Line 61: Line 61:
 Yes, xopX homologs can be found in almost every sequenced //Xanthomonas// spp. strain, except //X. albilineans// and //X. sacchari//, making it an ancient //Xanthomonas// core T3E (Stork //et al.//, 2015). Yes, xopX homologs can be found in almost every sequenced //Xanthomonas// spp. strain, except //X. albilineans// and //X. sacchari//, making it an ancient //Xanthomonas// core T3E (Stork //et al.//, 2015).
  
-**In other plant pathogens/symbionts **+**In other plant pathogens/symbionts**
  
 Related proteins (query cover > 80% and percent identity > 50 %) can be detected in several unclassified Burkholderiales (//Xylophilus ampelinus, Rivibacter// sp., //Rhizobacter// sp., //Mitsuaria// sp.) and in the Comamonadaceae (//Hydrogenophaga taeniospiralis//). Related proteins (query cover > 80% and percent identity > 50 %) can be detected in several unclassified Burkholderiales (//Xylophilus ampelinus, Rivibacter// sp., //Rhizobacter// sp., //Mitsuaria// sp.) and in the Comamonadaceae (//Hydrogenophaga taeniospiralis//).
bacteria/t3e/xopx.1753394042.txt.gz · Last modified: 2025/07/24 22:54 by jfpothier

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki