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--- bacteria:t3e:xopx [2025/02/13 12:52] – jfpothier
+++ bacteria:t3e:xopx [2025/02/13 15:54] (current) – [The Type III Effector XopX from //Xanthomonas//] rkoebnik
@@ Line 2: / Line 2: @@
 Author: [[https://www.researchgate.net/profile/Lucas_Moriniere|Lucas Morinière]] & [[https://www.researchgate.net/profile/Sohini_Deb|Sohini Deb]]\\
-Internal reviewer: Coline Sciallano\\
+Internal reviewer: [[https://www.researchgate.net/profile/Coline-Sciallano|Coline Sciallano]]\\
-Expert reviewer: Ramesh V. Sonti
+Expert reviewer: [[https://www.researchgate.net/profile/Ramesh_Sonti|Ramesh V. Sonti]]
 Class: XopX\\
@@ Line 16: / Line 16: @@
 === How discovered? ===
-XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// strain GM98-38 conjugated in //X. campestris// pv. //campestris// that allowed //Xcc// to elicit an //Xcv // cell death-like response when inoculated on //N. benthamiana// (Metz // et al//., 2005).
+XopX was discovered through the screening of a genomic cosmid library of //X. euvesicatoria// strain GM98-38 conjugated in //X. campestris// pv. //campestris// that allowed //Xcc// to elicit an //Xcv// cell death-like response when inoculated on //N. benthamiana// (Metz //et al//., 2005).
 === (Experimental) evidence for being a T3E ===
@@ Line 31: / Line 31: @@
   * When expressed in //Pseudomonas fluorescens// 55 (//Pf//55), a non‐pathogenic bacterium capable of eliciting PTI (callose deposit) in //Arabidopsis// Col‐0 plants, XopX is not able to reduce callose deposit, suggesting in these conditions, the effector is not able to suppress PTI. Using the same heterologuous system, XopX is not able to suppress the HR triggered by Pf 55 HopA1 on tobacco, suggesting that is these conditions, XopX do not act as an ETI suppressor. (Medina //et al//., 2018).
   * When transiently expressed in //N. benthamiana// by //Agrobacterium tumefaciens//–mediated expression system, XopX from //X. oryzae// pv. //oryzicola// cause the nonhost HR at approximately 2 days (Li //et al., //2015).
-  * A ∆//xopX// mutant strain of //Xanthomonas phaseoli// pv. //manihotis// (aka //Xanthomonas axonopodis// pv. //manihotis//) showed reduced growth //in planta// and delayed spread through the vasculature system of cassava (Mutka et al.//, 2016). //
+  * A ∆//xopX// mutant strain of //Xanthomonas phaseoli// pv. //manihotis// (aka //Xanthomonas axonopodis// pv. //manihotis//) showed reduced growth //in planta// and delayed spread through the vascular system of cassava (Mutka //et al.//, 2016).
-  * //Agrobacterium//-mediated transient expression of both //Xanthomonas oryzae // pv. //oryzae// XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).
+  * //Agrobacterium//-mediated transient expression of both //Xanthomonas oryzae// pv. //oryzae// XopQ and XopX in rice cells resulted in induction of rice immune responses. These immune responses were not observed when either protein was individually expressed in rice cells. XopQ-XopX induced rice immune responses were not observed with a XopX mutant that is defective in 14-3-3 binding (Deb //et al.//, 2020).
   * A screen for //Xanthomonas oryzae// pv. //oryzae// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
   *(ACD57163) is one among the five classes of virulence genes found to be upregulated in the //Xanthomonas oryzae// pv. //oryzae// MAI1 strain during infection. //xopX// was up-regulated at both 3 and 6 dai (Soto-Suárez //et al.//, 2010)
-  *The //xopX// gene contributes to the virulence of //Xanthomonas campestris// pv. //vesicatoria //on hosts pepper and tomato. XopX targets the innate immune response, resulting in enhanced plant disease susceptibility (Metz //et al//., 2005).
+  *The //xopX// gene contributes to the virulence of //Xanthomonas campestris// pv. //vesicatoria// on hosts pepper and tomato. XopX targets the innate immune response, resulting in enhanced plant disease susceptibility (Metz //et al//., 2005).
-  *A //Xanthomonas campestris//pv. //vesicatoria xopX// mutant strain could not induce cell death response in //N. benthamiana//, and could be complemented back to cell death response on //n. benthamiana// in trans with plasmid subclones of //xopX// (Metz //et al//., 2005).
+  *A //Xanthomonas campestris//pv. //vesicatoria xopX// mutant strain could not induce cell death response in //N. benthamiana//, and could be complemented back to cell death response on //N. benthamiana// in trans with plasmid subclones of //xopX// (Metz //et al//., 2005).
 === Localization ===
@@ Line 80: / Line 80: @@
 Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of //Xanthomonas campestris// pv. //vesicatoria// type III effectors in yeast affects cell growth and viability. Mol. Plant. Microbe Interact. 24: 305-314. DOI: [[https://doi.org/10.1094/MPMI-09-10-0196|10.1094/MPMI-09-10-0196]]
-Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of //Xanthomonas oryzae //pv. //oryzae//. PLoS One 8: e75867. DOI: [[https://doi.org/10.1371/journal.pone.0075867|10.1371/journal.pone.0075867]]
+Sinha D, Gupta MK, Patel HK, Ranjan A, Sonti RV (2013). Cell wall degrading enzyme induced rice innate immune responses are suppressed by the type 3 secretion system effectors XopN, XopQ, XopX and XopZ of //Xanthomonas oryzae// pv. //oryzae//. PLoS One 8: e75867. DOI: [[https://doi.org/10.1371/journal.pone.0075867|10.1371/journal.pone.0075867]]
-Soto-Suárez M, Bernal D, González C, Szurek B, Guyot R, Tohme J, Verdier V. //In planta// gene expression analysis of //Xanthomonas oryzae //pathovar //oryzae//, African strain MAI1. BMC Microbiol. 2010 Jun 11;10:170. DOI: [[https://doi.org/10.1186/1471-2180-10-170|10.1186/1471-2180-10-170]].
+Soto-Suárez M, Bernal D, González C, Szurek B, Guyot R, Tohme J, Verdier V. //In planta// gene expression analysis of //Xanthomonas oryzae// pathovar //oryzae//, African strain MAI1. BMC Microbiol. 2010 Jun 11;10:170. DOI: [[https://doi.org/10.1186/1471-2180-10-170|10.1186/1471-2180-10-170]].
 Stork W, Kim JG, Mudgett MB (2015). Functional analysis of plant defense suppression and activation by the //Xanthomonas// core type III effector XopX. Mol. Plant. Microbe Interact. 28: 180-194. DOI: [[https://doi.org/10.1094/MPMI-09-14-0263-R|10.1094/MPMI-09-14-0263-R]]

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