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bacteria:t3e:xopw

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bacteria:t3e:xopw [2025/02/24 11:52] – [Biological function] rkoebnikbacteria:t3e:xopw [2025/07/04 23:47] (current) – jfpothier
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 ====== The Type III Effector XopW from //Xanthomonas// ====== ====== The Type III Effector XopW from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopW\\ Class: XopW\\
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 XopW was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al//., 2009). XopW was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al//., 2009).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 TT3S-dependent translocation of XopW has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al//., 2009). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). TT3S-dependent translocation of XopW has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al//., 2009). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
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 === Regulation === === Regulation ===
  
bacteria/t3e/xopw.txt · Last modified: 2025/07/04 23:47 by jfpothier

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