EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopu

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
bacteria:t3e:xopu [2024/12/16 14:29] – [The Type III Effector XopU from //Xanthomonas//] rkoebnikbacteria:t3e:xopu [2025/07/04 23:46] (current) – jfpothier
Line 1: Line 1:
 ====== The Type III Effector XopU from //Xanthomonas// ====== ====== The Type III Effector XopU from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopU\\ Class: XopU\\
Line 15: Line 15:
  
 XopU was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al//., 2009). XopU was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al//., 2009).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 TT3S-dependent translocation of XopU has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al//., 2009). TT3S-dependent translocation of XopU has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al//., 2009).
 +
 === Regulation === === Regulation ===
  
Line 23: Line 25:
  
 //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020). //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
 +
 === Localization === === Localization ===
  
bacteria/t3e/xopu.1734359398.txt.gz · Last modified: 2024/12/16 14:29 by rkoebnik

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki