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--- bacteria:t3e:xopu [2020/07/17 09:36] – [Biological function] rkoebnik
+++ bacteria:t3e:xopu [2024/12/16 14:29] (current) – [The Type III Effector XopU from //Xanthomonas//] rkoebnik
@@ Line 1: / Line 1: @@
-====== XopU ======
+====== The Type III Effector XopU from //Xanthomonas// ======
-Author: John Doe\\
+Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]
-Reviewer: Jane Doe
 Class: XopU\\
 Family: XopU\\
-Prototype: Xop? (//Xanthomonas//; strain)\\
+Prototype: XOO2877 (//Xanthomonas oryzae// pv. //oryzae//; strain T7174)\\
-RefSeq ID:\\
+GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/BAE69632.1|BAE69632.1]] (985 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011408937.1|WP_011408937.1]] (985 aa)\\
 D structure: Unknown
@@ Line 14: / Line 14: @@
 === How discovered? ===
+XopU was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al//., 2009).
 === (Experimental) evidence for being a T3E ===
+TT3S-dependent translocation of XopU has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al//., 2009).
 === Regulation ===

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