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bacteria:t3e:xopq [2025/02/13 12:47] – jfpothierbacteria:t3e:xopq [2025/11/24 14:24] (current) – jfpothier
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 ====== The Type III Effector XopQ from //Xanthomonas// ====== ====== The Type III Effector XopQ from //Xanthomonas// ======
  
-Author: Valérie Olivier & Tamara Popović\\ +Author: Valérie Olivier, [[https://orcid.org/0000-0002-5789-7897|Tamara Popović]]\\ 
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopQ\\ Class: XopQ\\
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 XopQ was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopQ::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopQ was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_3177 (Jiang //et al.//, 2009). XopQ was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopQ::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopQ was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_3177 (Jiang //et al.//, 2009).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al//., 2004). Using an AvrBs1 reporter fusion, XopQ<sub>Xcc8004</sub> was shown to be translated into plant cells in a //hrpF//- and //hpaB//-dependent manner Jiang et al., 2009). Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al//., 2004). Using an AvrBs1 reporter fusion, XopQ<sub>Xcc8004</sub> was shown to be translated into plant cells in a //hrpF//- and //hpaB//-dependent manner Jiang et al., 2009).
 +
 === Regulation === === Regulation ===
  
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 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>, but this did not apply to //xopQ// (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>, but this did not apply to //xopQ// (Liu //et al.//, 2016).
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 === Phenotypes === === Phenotypes ===
  
bacteria/t3e/xopq.1739450856.txt.gz · Last modified: 2025/02/13 12:47 by jfpothier

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