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bacteria:t3e:xopp [2025/07/04 23:44] – jfpothierbacteria:t3e:xopp [2025/07/28 15:16] (current) – [Biological function] rkoebnik
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 XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009). XopP was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopP::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopP was also identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2994 (Jiang //et al.//, 2009).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). Using an AvrBs1 reporter fusion, XopP<sub>Xcc8004</sub> was shown to be translated into plant cells in a //hrpF//- and //hpaB//-dependent manner (Jiang //et al.//, 2009). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). +Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). Using an AvrBs1 reporter fusion, XopP<sub>Xcc8004</sub> was shown to be translated into plant cells in a //hrpF//- and //hpaB//-dependent manner (Jiang //et al.//, 2009). XopP<sub>//Xoo//13751</sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 === Regulation === === Regulation ===
  
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 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopP//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopP//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  (Liu //et al.//, 2016).
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 === Phenotypes === === Phenotypes ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//e.g.//, //X. campestris//, //X. citri//, //X. euvesicatoria//, //X. oryzae//, //X. translucens//). Since the G+C content of the //xopP//  gene is similar to that of the //Xcv//  //hrp//  gene cluster, it may be a member of a “core” group of //Xanthomonas//  spp. effectors (Roden et al., 2004).+Yes (//e.g.//, //X. campestris//, //X. citri//, //X. euvesicatoria//, //X. oryzae//, //X. translucens//). Since the G+C content of the //xopP// gene is similar to that of the //Xcv// //hrp// gene cluster, it may be a member of a “core” group of //Xanthomonas// spp. effectors (Roden et al., 2004).
  
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
bacteria/t3e/xopp.1751669044.txt.gz · Last modified: 2025/07/04 23:44 by jfpothier

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