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bacteria:t3e:xopo

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bacteria:t3e:xopo [2025/02/13 12:41] – jfpothierbacteria:t3e:xopo [2025/07/04 23:43] (current) – jfpothier
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 Author: Harrold van den Burg\\ Author: Harrold van den Burg\\
 Internal reviewer: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\
-Expert reviewer: Zoe Dubrow+Expert reviewer: [[https://www.researchgate.net/profile/Zoe_Dubrow|Zoe Dubrow]]\\
  
 Class: XopO\\ Class: XopO\\
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 XopO was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopO::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopO was identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//)strain 85-10. The XopO::AvrBs2 fusion protein triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004).
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 === Regulation === === Regulation ===
  
 XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006). XopO was found to be regulated by HrpG using HrpG* (Roden //et al//., 2004). //XopO// contains a PIP box sequence 31bp upstream of the -10 promoter motif (Koebnik //et al//., 2006).
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 === Phenotypes === === Phenotypes ===
  
bacteria/t3e/xopo.1739450513.txt.gz · Last modified: 2025/02/13 12:41 by jfpothier

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