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bacteria:t3e:xopn

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bacteria:t3e:xopn [2025/02/24 11:51] – [Biological function] rkoebnikbacteria:t3e:xopn [2026/06/22 10:25] (current) – [References] rkoebnik
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 Author: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\ Author: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Joana_Vicente2|Joana G. Vicente]]+Internal reviewer: [[https://www.researchgate.net/profile/Joana_Vicente2|Joana G Vicente]]\\
  
 Class: XopN\\ Class: XopN\\
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).+Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). XopN<sub>//Xoo//13751</sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 === Regulation === === Regulation ===
  
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   * //Agrobacterium//  mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae//  pv. //oryzae//  (Xoo), but a //xopN// <sup>//-// </sup>   mutant of //Xoo//retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; XopQ, XopX and XopZ were shown to be suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ//  genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae//  pv. //oryzae//  (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).   * //Agrobacterium//  mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae//  pv. //oryzae//  (Xoo), but a //xopN// <sup>//-// </sup>   mutant of //Xoo//retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; XopQ, XopX and XopZ were shown to be suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ//  genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae//  pv. //oryzae//  (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).
   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).
 +  * XopN<sub>//Xoc// </sub>  acts as a critical suppressor of rice basal immunity, perhaps by disrupting the formation of the OsRACK1B-OsRap2.6 immune module (Wang //et al.//, 2026).
  
 === Localization === === Localization ===
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 Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN<sub>KXO85</sub>  by yeast two-hybrid screening (Cheong et al., 2012). Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN<sub>KXO85</sub>  by yeast two-hybrid screening (Cheong et al., 2012).
 +
 +Using yeast two-hybrid screening, bimolecular fluorescence complementation, and pull-down assays, the WD40-repeat scaffold protein OsRACK1B was identified as a direct target of XopN<sub>//Xoc// </sub>  (Wang //et al.//, 2026).
  
 ===== Conservation ===== ===== Conservation =====
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 Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate //Xanthomonas// virulence. PLoS Pathog. 8: e1002768. DOI: [[https://doi.org/10.1371/journal.ppat.1002768|10.1371/journal.ppat.1002768]] Taylor KW, Kim JG, Su XB, Aakre CD, Roden JA, Adams CM, Mudgett MB (2012). Tomato TFT1 is required for PAMP-triggered immunity and mutations that prevent T3S effector XopN from binding to TFT1 attenuate //Xanthomonas// virulence. PLoS Pathog. 8: e1002768. DOI: [[https://doi.org/10.1371/journal.ppat.1002768|10.1371/journal.ppat.1002768]]
 +
 +Wang J, Ni Z, Chen X, Zhang Y, Qin H, Wang H, He Y, Tang J, Huang S (2026). //Xanthomonas// type III effector XopN targets scaffold protein OsRACK1B to suppress rice immunity. Mol. Plant Pathol. 27: e70300. DOI: [[https://doi.org/10.1111/mpp.70300|10.1111/mpp.70300]]
  
 Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]] Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]
bacteria/t3e/xopn.1740397868.txt.gz · Last modified: 2025/02/24 11:51 by rkoebnik

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