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bacteria:t3e:xopn [2025/02/24 11:50] – [References] rkoebnikbacteria:t3e:xopn [2025/12/11 12:25] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\ Author: [[https://www.researchgate.net/profile/Jakub_Pecenka|Jakub Pečenka]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Joana_Vicente2|Joana G. Vicente]]+Internal reviewer: [[https://www.researchgate.net/profile/Joana_Vicente2|Joana G Vicente]]\\
  
 Class: XopN\\ Class: XopN\\
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004).+Type III-dependent secretion was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). XopN<sub>//Xoo//13751</sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 === Regulation === === Regulation ===
  
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   * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// pv. //punicae// (//Xap//) in pomegranate (Kumar and Mondal, 2013). The deletion of XopN from Xap caused higher accumulation of reactive oxygen species showing that XopN suppresses ROS-mediated defense responses during blight pathogenesis in pomegranate (Kumar //et al.//, 2016).   * XopN has been shown to be required for maximal pathogenicity of //X. axonopodis// pv. //punicae// (//Xap//) in pomegranate (Kumar and Mondal, 2013). The deletion of XopN from Xap caused higher accumulation of reactive oxygen species showing that XopN suppresses ROS-mediated defense responses during blight pathogenesis in pomegranate (Kumar //et al.//, 2016).
   * A Δ//xopN//–Δ//xopQ// double knock-out mutant in //X. phaseoli// pv. //manihotis// (//Xpm//) was less aggressive in the cassava host plant than its single mutation counterparts. In addition, //in planta// bacterial growth was reduced at 5 dpi in the double mutant with respect to the wild-type strain CIO151 and individual knock-out strains. The phenotype of the double mutant could be complemented when transforming a plasmid containing //xopQ//. These results confirmed that //xopN// and //xopQ //are functionally redundant in //Xpm// (Medina //et al.//, 2017).   * A Δ//xopN//–Δ//xopQ// double knock-out mutant in //X. phaseoli// pv. //manihotis// (//Xpm//) was less aggressive in the cassava host plant than its single mutation counterparts. In addition, //in planta// bacterial growth was reduced at 5 dpi in the double mutant with respect to the wild-type strain CIO151 and individual knock-out strains. The phenotype of the double mutant could be complemented when transforming a plasmid containing //xopQ//. These results confirmed that //xopN// and //xopQ //are functionally redundant in //Xpm// (Medina //et al.//, 2017).
-  * //Agrobacterium// mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae// pv. //oryzae// (Xoo), but a //xopN// <sup>//-// </sup> mutant of //Xoo//retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; XopQ, XopX and XopZ were shown to be suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ// genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae// pv. //oryzae// (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).+  * //Agrobacterium// mediated transient transfer of the gene for XopN resulted in suppression of rice innate immune responses induced by LipA, a hydrolitic enzyme secreted by //X. oryzae// pv. //oryzae// (Xoo), but a //xopN// <sup>//-//</sup> mutant of //Xoo//retains the ability to suppress these innate immune responses indicating other functionally redundant proteins; XopQ, XopX and XopZ were shown to be suppressors of LipA induced innate immune responses; mutation in any one of the //xopN, xopQ, xopX or xopZ//  genes causes partial virulence deficiency (Sinha et al., 2013). XopN was shown to contribute significantly to //X. oryzae// pv. //oryzae// (Xoo) virulence on a susceptible rice variety Nipponbare. XopN was shown to be highly translocated to suppress rice defense responses (Mo //et al.//, 2020).
   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae// pv. //oryzicola// (Xoc GX01) (Liao //et al.//, 2020).   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae// pv. //oryzicola// (Xoc GX01) (Liao //et al.//, 2020).
  
bacteria/t3e/xopn.1740397840.txt.gz · Last modified: 2025/02/24 11:50 by rkoebnik

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