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bacteria:t3e:xopn [2026/10/29 14:52] – [References] rkoebnikbacteria:t3e:xopn [2026/10/29 15:15] (current) – [Biological function] rkoebnik
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 Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Jiang //et al//., 2008; Cheong //et al//., 2013). Start codon of //xopN// was found downstream of a conserved cis-regulatory element, the plant-inducible promoter (PIP) box (TTCGG-N15-TTCTG). //xopN// is regulated by //hrpX// and //hrpG// genes (Jiang //et al//., 2008; Cheong //et al//., 2013).
  
-qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> , but this did not apply to //xopN// (Liu //et al.//, 2016).+qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//) were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup>  , but this did not apply to //xopN// (Liu //et al.//, 2016).
 === Phenotypes === === Phenotypes ===
  
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   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).   * XopN and AvrBS2 were shown to significantly contribute to virulence of //X. oryzae//  pv. //oryzicola//  (Xoc GX01) (Liao //et al.//, 2020).
   * XopN<sub>//Xoc// </sub>  acts as a critical suppressor of rice basal immunity, perhaps by disrupting the formation of the OsRACK1B-OsRap2.6 immune module (Wang //et al.//, 2026).   * XopN<sub>//Xoc// </sub>  acts as a critical suppressor of rice basal immunity, perhaps by disrupting the formation of the OsRACK1B-OsRap2.6 immune module (Wang //et al.//, 2026).
 +  * Deletion of //xopN<sub>Xcc</sub> //  increased disease indices by more than 20% in cabbage line G1180, while bacterial counts also increased. In cabbage line G87-534, deletion of //xopN//  reduced both disease indices and bacterial counts (Chen //et al.//, 2026).
 +  * Transient expression of XopN<sub>//Xcc// </sub>  triggered a hypersensitive response in cabbage, and reverse transcription quantitative PCR (RT-qPCR) analyses revealed that XopN suppressed the expression of pathogenesis-related protein 1 (PR1) and WRKY transcription factor genes (WRKY18, WRKY33) associated with pattern-triggered immunity (Chen //et al.//, 2026)
  
 === Localization === === Localization ===
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 Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN<sub>KXO85</sub>  by yeast two-hybrid screening (Cheong et al., 2012). Two rice proteins, OsVOZ2 and a putative thiamine synthase (OsXNP) were identified as targets of XopN<sub>KXO85</sub>  by yeast two-hybrid screening (Cheong et al., 2012).
  
-Using yeast two-hybrid screening, bimolecular fluorescence complementation, and pull-down assays, the WD40-repeat scaffold protein OsRACK1B was identified as a direct target of XopN<sub>//Xoc// </sub>  (Wang //et al.//, 2026).+Using yeast two-hybrid screening, bimolecular fluorescence complementation, and pull-down assays, the WD40-repeat scaffold protein OsRACK1B was identified as a direct target of XopN<sub>//Xoc//  </sub>  (Wang //et al.//, 2026).
  
 ===== Conservation ===== ===== Conservation =====
bacteria/t3e/xopn.txt · Last modified: 2026/10/29 15:15 by rkoebnik

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