bacteria:t3e:xopm
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| bacteria:t3e:xopm [2024/12/17 10:05] – [The Type III Effector XopM from //Xanthomonas//] rkoebnik | bacteria:t3e:xopm [2025/07/04 23:42] (current) – jfpothier | ||
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| Author: [[https:// | Author: [[https:// | ||
| - | Expert reviewer: | + | Expert reviewer: |
| Class: XopM\\ | Class: XopM\\ | ||
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| === How discovered? === | === How discovered? === | ||
| - | //xopM// < | + | //xopM// < |
| - | < | + | |
| === (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
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| To test for T3SS-dependent translocation into plant cells, //Xee// < | To test for T3SS-dependent translocation into plant cells, //Xee// < | ||
| + | |||
| === Regulation === | === Regulation === | ||
| Expression of //xopM// < | Expression of //xopM// < | ||
| + | |||
| === Phenotypes === | === Phenotypes === | ||
| - | The //Xee// < | + | The // |
| + | |||
| + | To identify defense reactions, mediated by //xopM//, leaves of pepper ECW, //Nicotiana benthamiana// | ||
| + | When ectopically expressed in plants, XopM supports growth of a non-pathogenic bacterial strain and dampens the production of reactive oxygen species, indicating its ability to suppress plant immunity (Brinkmann et al., 2024). The abilty to repress a flg22 induced ROS burst is independent of XopM binding to VAP but requires localization at the host cell plasma membrane (see below, Brinkmann et al., 2024). | ||
| - | To identify defense reactions, mediated by //xopM//, leaves of pepper ECW, //Nicotiana benthamiana// | ||
| === Localization === | === Localization === | ||
| - | Unknown. | + | XopM-GFP fusions have been shown to localize to the plant plasma membrane (PM) in //N. benthamiana// |
| === Enzymatic function === | === Enzymatic function === | ||
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| === Interaction partners === | === Interaction partners === | ||
| - | Unknown. | + | XopM interacts with vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) in an isoform specific manner. XopM displays two FFAT [two phenylalanines (FF) in an acidic tract (AT)] motifs that cooperatively mediate the interaction with VAP. Binding to VAP is not required for XopM's ability to suppress PTI, indicating that it has other virulence targets. //In planta// pull-down assays using XopM-GFP as a bait, suggest that it interacts with additional membrane components (Brinkmann //et al//., 2024). |
| ===== Conservation ===== | ===== Conservation ===== | ||
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| //xopM// is typically encoded next to the //hrp// gene cluster and considered a core effector gene in several // | //xopM// is typically encoded next to the //hrp// gene cluster and considered a core effector gene in several // | ||
| + | |||
| === In other plant pathogens/ | === In other plant pathogens/ | ||
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| Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel // | Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel // | ||
| + | |||
| + | ===== Acknowledgements ===== | ||
| + | |||
| + | This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology). | ||
bacteria/t3e/xopm.1734429929.txt.gz · Last modified: 2024/12/17 10:05 by rkoebnik
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