bacteria:t3e:xopk
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| bacteria:t3e:xopk [2025/02/21 13:29] – [Conservation] rkoebnik | bacteria:t3e:xopk [2025/07/24 22:40] (current) – jfpothier | ||
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| Author: [[https:// | Author: [[https:// | ||
| Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
| - | Expert reviewer: Rebecca Bart | + | Expert reviewer: Rebecca Bart\\ |
| Class: XopK\\ | Class: XopK\\ | ||
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| XopK was discovered as a putative T3E based on the presence of a plant-inducible promoter box-like sequence and a -10 box-like sequence (Furutani //et al//., 2006). | XopK was discovered as a putative T3E based on the presence of a plant-inducible promoter box-like sequence and a -10 box-like sequence (Furutani //et al//., 2006). | ||
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| === (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
| Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). Expression of XopK is HrpX-dependent (Furutani //et al//., 2006) and was observed to translocate using a Cya reporter system (Furutani //et al//., 2009). | Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). Expression of XopK is HrpX-dependent (Furutani //et al//., 2006) and was observed to translocate using a Cya reporter system (Furutani //et al//., 2009). | ||
| + | |||
| === Regulation === | === Regulation === | ||
| Preceded by both a PIP box and a -10 box-like motif (Schulze //et al//., 2012; Furutani //et al//., 2006). | Preceded by both a PIP box and a -10 box-like motif (Schulze //et al//., 2012; Furutani //et al//., 2006). | ||
| - | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopK//, were significantly reduced in the // | + | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopK//, were significantly reduced in the // |
| === Phenotypes === | === Phenotypes === | ||
| - | * Deletion of XopK has been shown not to affect the virulence of //X. oryzae// | + | * Deletion of XopK has been shown not to affect the virulence of //X. oryzae// pv. // |
| - | * A ∆// | + | * A ∆//xopK// mutant strain of // |
| - | * XopK inhibits pathogen-associated molecular pattern-triggered immunity upstream of mitogen-activated protein kinase cascades (Qin //et al.//, 2018) | + | * XopK< |
| + | * Transgenic strawberries expressing XopK exhibit increased susceptibility to // | ||
| === Localization === | === Localization === | ||
| Line 37: | Line 41: | ||
| === Enzymatic function === | === Enzymatic function === | ||
| - | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // | + | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // |
| === Interaction partners === | === Interaction partners === | ||
| - | XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation (Qin //et al//., 2018) | + | XopK< |
| ===== Conservation ===== | ===== Conservation ===== | ||
| Line 47: | Line 51: | ||
| === In xanthomonads === | === In xanthomonads === | ||
| - | Yes (based on EDGAR [[https:// | + | Yes (based on EDGAR [[https:// |
| === In other plant pathogens/ | === In other plant pathogens/ | ||
| Line 54: | Line 59: | ||
| ===== References ===== | ===== References ===== | ||
| - | Cai XL, Zhang W, Yu H, Wen YQ, Feng JY (2024). The // | + | Cai XL, Zhang W, Yu H, Wen YQ, Feng JY (2024). The // |
| - | < | + | |
| Furutani A, Nakayama T, Ochiai H, Kaku H, Kubo Y, Tsuge S (2006). Identification of novel HrpXo regulons preceded by two // | Furutani A, Nakayama T, Ochiai H, Kaku H, Kubo Y, Tsuge S (2006). Identification of novel HrpXo regulons preceded by two // | ||
bacteria/t3e/xopk.1740144561.txt.gz · Last modified: 2025/02/21 13:29 by rkoebnik
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