bacteria:t3e:xopk
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| bacteria:t3e:xopk [2023/05/22 14:06] – [XopK] rkoebnik | bacteria:t3e:xopk [2025/07/24 22:40] (current) – jfpothier | ||
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| - | ====== XopK ====== | + | ====== |
| - | Author: [[https:// | + | Author: [[https:// |
| Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
| - | Expert reviewer: Rebecca Bart | + | Expert reviewer: Rebecca Bart\\ |
| Class: XopK\\ | Class: XopK\\ | ||
| Line 17: | Line 17: | ||
| XopK was discovered as a putative T3E based on the presence of a plant-inducible promoter box-like sequence and a -10 box-like sequence (Furutani //et al//., 2006). | XopK was discovered as a putative T3E based on the presence of a plant-inducible promoter box-like sequence and a -10 box-like sequence (Furutani //et al//., 2006). | ||
| + | |||
| === (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
| Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). Expression of XopK is HrpX-dependent (Furutani //et al//., 2006) and was observed to translocate using a Cya reporter system (Furutani //et al//., 2009). | Mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of rice somatic receptor kinase 2 (OsSERK2) and compromised XopK-dependent virulence (Qin //et al//., 2018). Expression of XopK is HrpX-dependent (Furutani //et al//., 2006) and was observed to translocate using a Cya reporter system (Furutani //et al//., 2009). | ||
| + | |||
| === Regulation === | === Regulation === | ||
| Preceded by both a PIP box and a -10 box-like motif (Schulze //et al//., 2012; Furutani //et al//., 2006). | Preceded by both a PIP box and a -10 box-like motif (Schulze //et al//., 2012; Furutani //et al//., 2006). | ||
| - | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopK//, were significantly reduced in the // | + | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopK//, were significantly reduced in the // |
| === Phenotypes === | === Phenotypes === | ||
| - | * Deletion of XopK has been shown not to affect the virulence of //X. oryzae// | + | * Deletion of XopK has been shown not to affect the virulence of //X. oryzae// pv. // |
| - | * A ∆// | + | * A ∆//xopK// mutant strain of // |
| - | * XopK inhibits pathogen-associated molecular pattern-triggered immunity upstream of mitogen-activated protein kinase cascades (Qin //et al.//, 2018) | + | * XopK< |
| + | * Transgenic strawberries expressing XopK exhibit increased susceptibility to // | ||
| === Localization === | === Localization === | ||
| - | The XopK sequence contains 54% hydrophobic residues and several predicted transmembrane domains. Thus, it is possible this protein is associated with host cell membranes following secretion (Mutka //et al//., 2016) | + | The XopK sequence contains 54% hydrophobic residues and several predicted transmembrane domains. Thus, it is possible |
| === Enzymatic function === | === Enzymatic function === | ||
| - | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // | + | The protein has E3 ubiquinol ligase activity. The putative E2-binding site is highly conserved in the majority of members from different // |
| === Interaction partners === | === Interaction partners === | ||
| - | XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation (Qin //et al//., 2018) | + | XopK< |
| ===== Conservation ===== | ===== Conservation ===== | ||
| Line 47: | Line 51: | ||
| === In xanthomonads === | === In xanthomonads === | ||
| - | Yes (based on EDGAR; e.g., //X. oryzae// | + | Yes (based on EDGAR [[https:// |
| === In other plant pathogens/ | === In other plant pathogens/ | ||
| - | Yes (e.g., // | + | Yes (e.g., // |
| ===== References ===== | ===== References ===== | ||
| - | Furutani A, Nakayama T, Ochiai | + | Cai XL, Zhang W, Yu H, Wen YQ, Feng JY (2024). The //Xanthomonas fragariae// effector XopK suppresses stomatal immunity by perturbing abscisic acid accumulation |
| - | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// | + | Furutani A, Nakayama |
| - | Liu Y, Long J, Shen D, Song C (2016). // | + | Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in // |
| + | |||
| + | Liu Y, Long J, Shen D, Song C (2016). // | ||
| Mutka AM, Fentress SJ, Sher SW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | Mutka AM, Fentress SJ, Sher SW, Berry JC, Pretz C, Nusinow DA, Bart R (2016). Quantitative, | ||
| - | Qin J, Zhou X, Sun L, Wang K, Yang F, Liao H, Rong W, Yin J, Chen H, Chen X, Zhang J (2018). The // | + | Qin J, Zhou X, Sun L, Wang K, Yang F, Liao H, Rong W, Yin J, Chen H, Chen X, Zhang J (2018). The // |
| + | |||
| + | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | ||
| + | |||
| + | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | ||
| - | Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from // | + | ===== Acknowledgements ===== |
| - | Song C, Yang B (2010). Mutagenesis of 18 type III effectors reveals virulence function of XopZ< | + | This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology). |
bacteria/t3e/xopk.1684760801.txt.gz · Last modified: 2023/05/22 14:06 by rkoebnik
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