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bacteria:t3e:xopj8

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bacteria:t3e:xopj8 [2025/07/24 17:11] – [References] rkoebnikbacteria:t3e:xopj8 [2025/07/24 23:30] (current) – jfpothier
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 Author: Anna Passelergue\\ Author: Anna Passelergue\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 === How discovered? === === How discovered? ===
  
-XopJ8 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals at the N terminus (Thieme //et al.//, 2007). The protein was also predicted as a type III effector by [[https://effectidor.tau.ac.il/|Effectidor II]], a pan-genomic AI-based algorithm for the prediction of type III secretion system effectors (Wagner //et al.//, 2025). The prototype gene in //X. hyacinthi// strain CFBP 1156 is flanked by two IS//3//-family insertion sequences.+XopJ8 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals at the N terminus (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007). The protein was also predicted as a type III effector by [[https://effectidor.tau.ac.il/|Effectidor II]], a pan-genomic AI-based algorithm for the prediction of type III secretion system effectors (Wagner //et al.//, 2025). The prototype gene in //X. hyacinthi// strain CFBP 1156 is flanked by two IS//3//-family insertion sequences. 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 XopJ8 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). XopJ8 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 +
 === Regulation === === Regulation ===
  
 The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ8// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ8// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
 +
 === Phenotypes === === Phenotypes ===
  
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 === Localization === === Localization ===
  
-The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Thieme //et al.//, 2007).+The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007). 
 === Enzymatic function === === Enzymatic function ===
  
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 Yes (//e.g.//, //X. citri//, frameshifted versions of the gene exist in //X. graminis// pv. //graminis//). Yes (//e.g.//, //X. citri//, frameshifted versions of the gene exist in //X. graminis// pv. //graminis//).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xopj8.1753373516.txt.gz · Last modified: 2025/07/24 17:11 by rkoebnik

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