EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopj7

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
bacteria:t3e:xopj7 [2025/07/24 17:09] – [References] rkoebnikbacteria:t3e:xopj7 [2025/07/24 23:28] (current) – jfpothier
Line 2: Line 2:
  
 Author: Anna Passelergue\\ Author: Anna Passelergue\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
Line 15: Line 15:
 === How discovered? === === How discovered? ===
  
-XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals, which was in frame with the ORF. Based on this observation, the translation initiation codon is likely 104 codons upstream of the annotated codon for the locus E4A48_RS06415.+XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007), which were in frame with the ORF. Based on this observation, the translation initiation codon is likely 104 codons upstream of the annotated codon for the locus E4A48_RS06415. 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 +
 === Regulation === === Regulation ===
  
 The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
 +
 === Phenotypes === === Phenotypes ===
  
Line 28: Line 31:
 === Localization === === Localization ===
  
-The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Thieme //et al.//, 2007).+The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007).
 === Enzymatic function === === Enzymatic function ===
  
Line 42: Line 45:
  
 Yes (//e.g.//, //X. euvesicatoria//, //X. campestris//, //X. hortorum//). Yes (//e.g.//, //X. euvesicatoria//, //X. campestris//, //X. hortorum//).
-=== In other plant pathogens/symbionts ===+= 
 +== In other plant pathogens/symbionts ===
  
 Yes (e.g., //Acidovorax/////Paracidovorax// ssp., //Burkholderia/////Paraburkholderia// ssp; remote homologs in //Ralstonia// ssp.]. Yes (e.g., //Acidovorax/////Paracidovorax// ssp., //Burkholderia/////Paraburkholderia// ssp; remote homologs in //Ralstonia// ssp.].
bacteria/t3e/xopj7.1753373370.txt.gz · Last modified: 2025/07/24 17:09 by rkoebnik

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki