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bacteria:t3e:xopj7

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bacteria:t3e:xopj7 [2025/07/24 12:49] – [Biological function] rkoebnikbacteria:t3e:xopj7 [2025/07/24 23:28] (current) – jfpothier
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 Author: Anna Passelergue\\ Author: Anna Passelergue\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 === How discovered? === === How discovered? ===
  
-XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of a conserved palmitoylation signal, which was in frame with the ORF. Based on this observation, the translation initiation codon is likely 104 codons upstream of the annotated codon for the locus E4A48_RS06415.+XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007), which were in frame with the ORF. Based on this observation, the translation initiation codon is likely 104 codons upstream of the annotated codon for the locus E4A48_RS06415. 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 +
 === Regulation === === Regulation ===
  
 The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
 +
 === Phenotypes === === Phenotypes ===
  
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 === Localization === === Localization ===
  
-The presence of a conserved palmitoylation signal at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Thieme //et al.//, 2007).+The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007).
 === Enzymatic function === === Enzymatic function ===
  
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 Yes (//e.g.//, //X. euvesicatoria//, //X. campestris//, //X. hortorum//). Yes (//e.g.//, //X. euvesicatoria//, //X. campestris//, //X. hortorum//).
-=== In other plant pathogens/symbionts ===+= 
 +== In other plant pathogens/symbionts ===
  
 Yes (e.g., //Acidovorax/////Paracidovorax// ssp., //Burkholderia/////Paraburkholderia// ssp; remote homologs in //Ralstonia// ssp.]. Yes (e.g., //Acidovorax/////Paracidovorax// ssp., //Burkholderia/////Paraburkholderia// ssp; remote homologs in //Ralstonia// ssp.].
Line 49: Line 53:
  
 Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]] Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]]
 +
 +Nimchuk Z, Marois E, Kjemtrup S, Leister RT, Katagiri F, Dangl JL (2000). Eukaryotic fatty acylation drives plasma membrane targeting and enhances function of several type III effector proteins from //Pseudomonas syringae//. Cell 101: 353-363. DOI: [[https://doi.org/10.1016/s0092-8674(00)80846-6|10.1016/s0092-8674(00)80846-6]]
  
 Passelergue A (2025). Discovery of eight type III effector genes harboring the PIP box in clade-I xanthomonads. Master's thesis, Université de Montpellier, France. Passelergue A (2025). Discovery of eight type III effector genes harboring the PIP box in clade-I xanthomonads. Master's thesis, Université de Montpellier, France.
bacteria/t3e/xopj7.1753357761.txt.gz · Last modified: 2025/07/24 12:49 by rkoebnik

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