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bacteria:t3e:xopj7 [2025/07/24 10:05] – [References] rkoebnikbacteria:t3e:xopj7 [2025/07/24 23:28] (current) – jfpothier
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 Author: Anna Passelergue\\ Author: Anna Passelergue\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
 Family: XopJ7\\ Family: XopJ7\\
-Prototype: XTG_RS02340 (//Xanthomonas euroxanthea//, strain CPBF 424) Attention: The prototype sequence is too long because codons 18 to 39 overlap with the plant-inducible promoter (see below; Koebnik //et al.//, 2006).\\ +Prototype: E4A48_06420 (//Xanthomonas// //cerealis// pv. //cerealis//, strain 01)\\ 
-GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAE1133144.1|CAJ22212.1]] (286 aa)\\ +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/QDI05802.1|QDI05802.1]] (493 aa)\\ 
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_212580660.1|WP_039417318.1]] (216 aa)\\+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_185910725.1|WP_185910725.1]] (389 aa). Attention: The RefSeq sequence is too short because 104 codons encoding the N-terminal region, including the type III secretion signal, are missing.\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT). Additional evidence came from the presence of a conserved palmitoylation signal, which was in frame with the ORF.+XopJ7 was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025). Additional evidence came from the presence of conserved myristoylation and palmitoylation signals (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007), which were in frame with the ORF. Based on this observation, the translation initiation codon is likely 104 codons upstream of the annotated codon for the locus E4A48_RS06415. 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). XopJ7 was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 +
 === Regulation === === Regulation ===
  
 The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopJ7// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
 +
 === Phenotypes === === Phenotypes ===
  
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 === Localization === === Localization ===
  
-The presence of a conserved palmitoylation signal at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Thieme //et al.//, 2007).+The presence of conserved myristoylation and palmitoylation signals at the N terminus suggests that the protein is localized to the plasma membrane in the plant host cell (Nimchuk //et al.//, 2000; Thieme //et al.//, 2007).
 === Enzymatic function === === Enzymatic function ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//e.g.//, //X. arboricola//, //X. campestris// pv. //papavericola//, //X. hortorum//). +Yes (//e.g.//, //X. euvesicatoria//, //X. campestris//, //X. hortorum//). 
-=== In other plant pathogens/symbionts ===+= 
 +== In other plant pathogens/symbionts ===
  
-No.+Yes (e.g., //Acidovorax/////Paracidovorax// ssp., //Burkholderia/////Paraburkholderia// ssp; remote homologs in //Ralstonia// ssp.].
  
 ===== References ===== ===== References =====
  
 Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]] Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]]
 +
 +Nimchuk Z, Marois E, Kjemtrup S, Leister RT, Katagiri F, Dangl JL (2000). Eukaryotic fatty acylation drives plasma membrane targeting and enhances function of several type III effector proteins from //Pseudomonas syringae//. Cell 101: 353-363. DOI: [[https://doi.org/10.1016/s0092-8674(00)80846-6|10.1016/s0092-8674(00)80846-6]]
  
 Passelergue A (2025). Discovery of eight type III effector genes harboring the PIP box in clade-I xanthomonads. Master's thesis, Université de Montpellier, France. Passelergue A (2025). Discovery of eight type III effector genes harboring the PIP box in clade-I xanthomonads. Master's thesis, Université de Montpellier, France.
bacteria/t3e/xopj7.1753347958.txt.gz · Last modified: 2025/07/24 10:05 by rkoebnik

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