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bacteria:t3e:xopj6 [2025/01/27 23:07] – [The Type III Effector XopJ6 from //Xanthomonas//] jfpothierbacteria:t3e:xopj6 [2025/07/24 22:38] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Laurent-Noel-3|Laurent Noël]]\\ Author: [[https://www.researchgate.net/profile/Laurent-Noel-3|Laurent Noël]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 === How discovered? === === How discovered? ===
  
-XopJ6 was initially identified in //Xanthomonas campestris //pv. //campestris //as a homolog of //Ralstonia pseudosolanacearum //, the presence of which correlated with induction of an hypersensitive response (HR) on //Brassacia oleracea// cv. clovis F1 (Lauber //et al//., 2024).+XopJ6 was initially identified in //Xanthomonas campestris// pv. //campestris// as a homolog of //Ralstonia pseudosolanacearum//, the presence of which correlated with induction of an hypersensitive response (HR) on //Brassacia oleracea// cv. clovis F1 (Lauber //et al//., 2024). 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
   * XopJ6 is a homolog of PopP2, with 35% overall sequence identity with a good conservation of the acetyltransferase domain (Lauber //et al//., 2024).   * XopJ6 is a homolog of PopP2, with 35% overall sequence identity with a good conservation of the acetyltransferase domain (Lauber //et al//., 2024).
-  * //xopJ6//  induces HR on cauliflower in a type III secretion-dependent-manner (Lauber //et al//., 2024).+  * //xopJ6// induces HR on cauliflower in a type III secretion-dependent-manner (Lauber //et al//., 2024).
   * In Arabidopsis, XopJ6 effector-triggered immunity (ETI) is mediated by two nuclear proteins RRS1 and RPS4 (Lauber //et al//., 2024).   * In Arabidopsis, XopJ6 effector-triggered immunity (ETI) is mediated by two nuclear proteins RRS1 and RPS4 (Lauber //et al//., 2024).
   * No direct experimental demonstration for translocation.   * No direct experimental demonstration for translocation.
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 === Genomic organization === === Genomic organization ===
  
-//xopJ6//  is present on a ca. 9-kb transposable element named Tn//6714//. It was found in multiple identical copies, likely due to recent transposition events (Lauber //et al//., 2024). Many //Xanthomonas campestris //pv. //campestris //strains do not contain any// xopJ6 //gene and are still potent and adapted pathogenic strains (Lauber //et al//., 2024).+//xopJ6// is present on a ca. 9-kb transposable element named Tn//6714//. It was found in multiple identical copies, likely due to recent transposition events (Lauber //et al//., 2024). Many //Xanthomonas campestris// pv. //campestris// strains do not contain any //xopJ6//gene and are still potent and adapted pathogenic strains (Lauber //et al//., 2024).
  
 === Regulation === === Regulation ===
  
-//xopJ6//  promoters possess a PIP-box motif (Lauber //et al//., 2024). Expression of //xopJ6//  is induced in the //hrp//-inducing medium MME or by the //hrpG//* gain-of-function allele of //hrpG//  in a //hrpG//- and //hrpX//-dependent manner (Lauber //et al//., 2024). Copy number variation of //xopJ6//  results in variable levels of transcription.+//xopJ6// promoters possess a PIP-box motif (Lauber //et al//., 2024). Expression of //xopJ6// is induced in the //hrp//-inducing medium MME or by the //hrpG//* gain-of-function allele of //hrpG// in a //hrpG//- and //hrpX//-dependent manner (Lauber //et al//., 2024). Copy number variation of //xopJ6// results in variable levels of transcription.
  
 === Phenotypes === === Phenotypes ===
  
-  * //xopJ6//  induces an hypersensitive response and ETI on the cauliflower (Brassica oleracea) cv. Clovis F1 (Lauber //et al//., 2024). +  * //xopJ6// induces an hypersensitive response and ETI on the cauliflower (//Brassica oleracea//) cv. Clovis F1 (Lauber //et al//., 2024). 
-  * //xopJ6//  induces ETI in //Arabidopsis thaliana//  in an RRS1-R- and RPS4-dependent manner (Lauber //et al//., 2024). +  * //xopJ6// induces ETI in //Arabidopsis thaliana// in an RRS1-R- and RPS4-dependent manner (Lauber //et al//., 2024). 
-  * //xopJ6 //delivered by //Pseudomonas fluorescens//  Pfo-1 induces HR in //Arabidopsis thaliana//  in an RRS1-R-, EDS1- and RPS4-dependent manner (Lauber //et al//., 2024). +  * //xopJ6// delivered by //Pseudomonas fluorescens// Pfo-1 induces HR in //Arabidopsis thaliana// in an RRS1-R-, EDS1- and RPS4-dependent manner (Lauber //et al//., 2024). 
-  * //xopJ6//  is able to dampen flg22-induced PTI responses in //Nicotiana benthamiana //  (Lauber //et al//., 2024)//.// +  * //xopJ6// is able to dampen flg22-induced PTI responses in //Nicotiana benthamiana// (Lauber //et al//., 2024)//.// 
-  * //xopJ6//  quantitatively contributes to //Xanthomonas campestris//  pv. //campestris//  pathogenicity in susceptible accessions Col-0 and Sf-2 of Arabidopsis: //xopJ6//  mutants are less virulent while those overexpressing //xopJ6//  are more virulent (Lauber //et al//., 2024).+  * //xopJ6// quantitatively contributes to //Xanthomonas campestris// pv. //campestris// pathogenicity in susceptible accessions Col-0 and Sf-2 of Arabidopsis: //xopJ6// mutants are less virulent while those overexpressing //xopJ6// are more virulent (Lauber //et al//., 2024). 
 === Localization === === Localization ===
  
-XopJ6-CFP has a nucleo-cytoplasmic localization upon //Agrobacterium//-mediated expression in //N. benthamiana//  (Lauber //et al//., 2024).+XopJ6-CFP has a nucleo-cytoplasmic localization upon //Agrobacterium//-mediated expression in //N. benthamiana// (Lauber //et al//., 2024).
  
 === Enzymatic function === === Enzymatic function ===
  
-Recombinant XopJ6 purified from //E. coli //encodes an acetyl transferase able to acetylate Arabidopsis WRKY22 (Lauber //et al//., 2024).+Recombinant XopJ6 purified from //E. coli// encodes an acetyl transferase able to acetylate Arabidopsis WRKY22 (Lauber //et al//., 2024).
  
 === Interaction partners === === Interaction partners ===
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   * XopJ6 interacts with Arabidopdis RRS1-R resistance protein and WRKY22 transcription factor (Lauber //et al//., 2024).   * XopJ6 interacts with Arabidopdis RRS1-R resistance protein and WRKY22 transcription factor (Lauber //et al//., 2024).
   * A single AA substituion (N382K) abolishes interaction with Arabidopsis WRKY22 and RRS1-R and is required for ETI in Arabidopsis and cauliflower (Lauber //et al//., 2024).   * A single AA substituion (N382K) abolishes interaction with Arabidopsis WRKY22 and RRS1-R and is required for ETI in Arabidopsis and cauliflower (Lauber //et al//., 2024).
-  * WT XopJ6 and XopJ6<sup>N382K</sup>   both suppress Flg22 ROS burst in a WRKY-independent manner (Lauber //et al//., 2024).+  * WT XopJ6 and XopJ6<sup>N382K</sup> both suppress Flg22 ROS burst in a WRKY-independent manner (Lauber //et al//., 2024).
  
 ===== Conservation ===== ===== Conservation =====
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 By 2024, xopJ6 has only been detected in //Xanthomonas campestris// pv. //campestris// (Lauber //et al//., 2024) and in //Xanthomonas translucens// pv. //graminis//. By 2024, xopJ6 has only been detected in //Xanthomonas campestris// pv. //campestris// (Lauber //et al//., 2024) and in //Xanthomonas translucens// pv. //graminis//.
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xopj6.1738019272.txt.gz · Last modified: 2025/01/27 23:07 by jfpothier

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