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--- bacteria:t3e:xopj6 [2024/04/25 10:41] – [Biological function] rkoebnik
+++ bacteria:t3e:xopj6 [2025/02/24 15:32] (current) – [The Type III Effector XopJ6 from //Xanthomonas//] rkoebnik
@@ Line 1: / Line 1: @@
-====== XopJ6 ======
+====== The Type III Effector XopJ6 from //Xanthomonas// ======
-Author: Laurent NOËL\\
+Author: [[https://www.researchgate.net/profile/Laurent-Noel-3|Laurent Noël]]\\
-Reviewer: Jane Doe
+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]
 Class: XopJ\\
 Family: XopJ6\\
-Prototype: XopJ6 (//Xanthomonas campestris //pv. //campestris//; strain CN06)\\
+Prototype: XopJ6 (//Xanthomonas campestris// pv. //campestris//; strain CN06)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_196765268.1|WP_196765268.1]] (471 aa)\\
 D structure: Unknown
@@ Line 14: / Line 14: @@
 === How discovered? ===
-XopJ6 was initially identified in //Xanthomonas campestris //pv. //campestris //as a homolog of //Ralstonia pseudosolanacearum //, the presence of which correlated with induction of an hypersensitive response (HR) on //Brassacia oleracea// cv. clovis F1 (Lauber //et al//., 2024).
+XopJ6 was initially identified in //Xanthomonas campestris// pv. //campestris// as a homolog of //Ralstonia pseudosolanacearum//, the presence of which correlated with induction of an hypersensitive response (HR) on //Brassacia oleracea// cv. clovis F1 (Lauber //et al//., 2024).
 === (Experimental) evidence for being a T3E ===
-XopJ6 is a homolog of PopP2, with 35% overall sequence identity with a good conservation of the acetyltransferase domain (Lauber //et al//., 2024).
+  * XopJ6 is a homolog of PopP2, with 35% overall sequence identity with a good conservation of the acetyltransferase domain (Lauber //et al//., 2024).
+  * //xopJ6//  induces HR on cauliflower in a type III secretion-dependent-manner (Lauber //et al//., 2024).
-//xopJ6// induces HR on cauliflower in a type III secretion-dependent-manner (Lauber //et al//., 2024).
+  * In Arabidopsis, XopJ6 effector-triggered immunity (ETI) is mediated by two nuclear proteins RRS1 and RPS4 (Lauber //et al//., 2024).
+  * No direct experimental demonstration for translocation.
-In Arabidopsis, XopJ6 ETI is medaited by two nuclear proteins RRS1 and RPS4 (Lauber //et al//., 2024).
-No direct experimental demonstration for translocation.
 === Genomic organization ===
-//xopJ6// in present in a ca. 9-kb transposable element named Tn6714. It can be found in multiple identical copies likely due to recent transposition events (Lauber //et al//., 2024).
+//xopJ6//  is present on a ca. 9-kb transposable element named Tn//6714//. It was found in multiple identical copies, likely due to recent transposition events (Lauber //et al//., 2024). Many //Xanthomonas campestris//  pv. //campestris//  strains do not contain any //xopJ6//gene and are still potent and adapted pathogenic strains (Lauber //et al//., 2024).
-Many //Xanthomonas campestris //pv. //campestris //strains do not contain any// xopJ6 //gene and are still potent and adapted pathogenic strains (Lauber //et al//., 2024).
 === Regulation ===
-//xopJ6// promoters possess a PIP-box motif (Lauber //et al//., 2024).
+//xopJ6//  promoters possess a PIP-box motif (Lauber //et al//., 2024). Expression of //xopJ6//  is induced in the //hrp//-inducing medium MME or by the //hrpG//* gain-of-function allele of //hrpG//  in a //hrpG//- and //hrpX//-dependent manner (Lauber //et al//., 2024). Copy number variation of //xopJ6//  results in variable levels of transcription.
-Expression of //xopJ6// is induced in the //hrp//-inducing medium MME or by the hrpG* gain-of-function allele of hrpG in a //hrpG//- and //hrpX//-dependent manner (Lauber //et al//., 2024).
-Copy number variation of //xopJ6// results in variable levels of transcription.
 === Phenotypes ===
-//xopJ6// induces an hypersensitive response and ETI on the cauliflower (Brassica oleracea) cv. Clovis F1 (Lauber //et al//., 2024).
+  * //xopJ6//  induces an hypersensitive response and ETI on the cauliflower (//Brassica oleracea//) cv. Clovis F1 (Lauber //et al//., 2024).
+  * //xopJ6//  induces ETI in //Arabidopsis thaliana//  in an RRS1-R- and RPS4-dependent manner (Lauber //et al//., 2024).
+  * //xopJ6//  delivered by //Pseudomonas fluorescens//  Pfo-1 induces HR in //Arabidopsis thaliana//  in an RRS1-R-, EDS1- and RPS4-dependent manner (Lauber //et al//., 2024).
+  * //xopJ6//  is able to dampen flg22-induced PTI responses in //Nicotiana benthamiana//  (Lauber //et al//., 2024)//.//
+  * //xopJ6//  quantitatively contributes to //Xanthomonas campestris//  pv. //campestris//  pathogenicity in susceptible accessions Col-0 and Sf-2 of Arabidopsis: //xopJ6//  mutants are less virulent while those overexpressing //xopJ6//  are more virulent (Lauber //et al//., 2024).
+=== Localization ===
-//xopJ6// induces ETI in //Arabidopsis thaliana// in an RRS1-R- and RPS4-dependent manner (Lauber //et al//., 2024).
+XopJ6-CFP has a nucleo-cytoplasmic localization upon //Agrobacterium//-mediated expression in //N. benthamiana//  (Lauber //et al//., 2024).
-//xopJ6 //delivered by //Pseudomonas fluorescens// Pfo-1 induces HR in //Arabidopsis thaliana// in an RRS1-R-, EDS1- and RPS4-dependent manner (Lauber //et al//., 2024).
+=== Enzymatic function ===
-//xopJ6// is able to dampen flg22-induced PTI responses in //Nicotiana benthamiana // (Lauber //et al//., 2024)//.//
+Recombinant XopJ6 purified from //E. coli//  encodes an acetyl transferase able to acetylate Arabidopsis WRKY22 (Lauber //et al//., 2024).
-//xopJ6// quantitatively contributes to //Xanthomonas campestris// pv. //campestris// pathogenicity in susceptible accession Col-0 or Sf-2Arabidopsis: //xopJ6// mutants are less virulent while those overexpressing //xopJ6// are more virulent (Lauber //et al//., 2024).
-=== Localization ===
-XopJ6-CFP has a nucleo-cytoplasmic localization upon //Agrobacterium//-mediated expression in //Nicotiana benthamiana// (Lauber //et al//., 2024).
-=== Enzymatic function ===
-Recombinant XopJ6 purified from //E. coli //encodes an acetyl transferase able to acetylate Arabidopsis WRKY22 (Lauber //et al//., 2024).
 === Interaction partners ===
-XopJ6 interacts with RRS1-R WRKY domain in yeast two-hybrid experiments (Lauber //et al//., 2024).
+  * XopJ6 interacts with RRS1-R WRKY domain in yeast two-hybrid experiments (Lauber //et al//., 2024).
+  * XopJ6 interacts with Arabidopdis RRS1-R resistance protein and WRKY22 transcription factor (Lauber //et al//., 2024).
+  * A single AA substituion (N382K) abolishes interaction with Arabidopsis WRKY22 and RRS1-R and is required for ETI in Arabidopsis and cauliflower (Lauber //et al//., 2024).
+  * WT XopJ6 and XopJ6<sup>N382K</sup>   both suppress Flg22 ROS burst in a WRKY-independent manner (Lauber //et al//., 2024).
-XopJ6 interacts with Arabidopdis RRS1-R resistance protein and WRKY22 transcription factor (Lauber //et al//., 2024).
-A single AA substituion (N382K) abolishes interaction with Arabidopsis WRKY22 and RRS1-R and is required for ETI in Arabidopsis and cauliflower (Lauber //et al//., 2024).
-WT XopJ6 and XopJ6<sup>N382K</sup>  both suppress Flg22 ROS burst in a WRKY-independent manner (Lauber //et al//., 2024).
 ===== Conservation =====
 === In xanthomonads ===
-By 2024, xopJ6 has only been detected in //Xanthomonas campestris// pv. //campestris// (Lauber //et al//., 2024) and in //Xanthomonas translucens// pv. //graminis//.
+By 2024, xopJ6 has only been detected in //Xanthomonas campestris//  pv. //campestris//  (Lauber //et al//., 2024) and in //Xanthomonas translucens//  pv. //graminis//.
 === In other plant pathogens/symbionts ===
-XopJ6 is a homolog of the type III effector PopP2 from //Rastonia pseudosolanacearum//. Homologs are also present in strains of //Collimonas// and //Duganella//.
+XopJ6 is a homolog of the type III effector PopP2 from //Rastonia pseudosolanacearum//. Homologs are also present in strains of //Collimonas//  and //Duganella//.
 ===== References =====

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