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bacteria:t3e:xopj5

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bacteria:t3e:xopj5 [2025/02/13 11:49] – jfpothierbacteria:t3e:xopj5 [2025/07/04 23:38] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Alexandre_Menezes6|Alexandre B. de Menezes]]\\ Author: [[https://www.researchgate.net/profile/Alexandre_Menezes6|Alexandre B. de Menezes]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Katarina_Gasic|Katarina Gašić]]+Internal reviewer: [[https://www.researchgate.net/profile/Katarina_Gasic|Katarina Gašić]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 In //Arabidopsis//, AvrXccB (aka XopJ5) targets putative S- adenosyl-L-methionine-dependent methyltransferase (SAM-MT) (Liu //et al//., 2017). In //Arabidopsis//, AvrXccB (aka XopJ5) targets putative S- adenosyl-L-methionine-dependent methyltransferase (SAM-MT) (Liu //et al//., 2017).
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 === How discovered? === === How discovered? ===
  
 Original discovery was through bioinformatic predictions and comparative genomics of //Xanthomonas axonopodis// pv. //citri// (//Xac//) and //Xanthomonas campestris// pv. //campestris// (//Xcc//) (da Silva //et al//., 2002). XopJ5 (AvrXccB) was also identified in //Xcc// strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_3802 (Jiang //et al.//, 2009). Original discovery was through bioinformatic predictions and comparative genomics of //Xanthomonas axonopodis// pv. //citri// (//Xac//) and //Xanthomonas campestris// pv. //campestris// (//Xcc//) (da Silva //et al//., 2002). XopJ5 (AvrXccB) was also identified in //Xcc// strain 8004 as a candidate T3E due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_3802 (Jiang //et al.//, 2009).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 AvrXccB, fused to a HA tag, was shown to be secreted into culture supernatants by Western blot analysis using an anti‐haemagglutinin (HA) antibody. In contrast, a T3E-defective mutant was not capable of secreting this effector (Liu //et al//., 2017). AvrXccB, fused to a HA tag, was shown to be secreted into culture supernatants by Western blot analysis using an anti‐haemagglutinin (HA) antibody. In contrast, a T3E-defective mutant was not capable of secreting this effector (Liu //et al//., 2017).
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 === Regulation === === Regulation ===
  
 The //xopE1// <sub>Xcc8004</sub> gene (//avrXccB//) contains a PIP box and was shown to be controlled by //hrpG// and //hrpX// (Rongqi //et al//., 2006; Jiang et al., 2009). The //xopE1// <sub>Xcc8004</sub> gene (//avrXccB//) contains a PIP box and was shown to be controlled by //hrpG// and //hrpX// (Rongqi //et al//., 2006; Jiang et al., 2009).
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 === Phenotypes === === Phenotypes ===
  
bacteria/t3e/xopj5.1739447382.txt.gz · Last modified: 2025/02/13 11:49 by jfpothier

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