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bacteria:t3e:xopj3

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bacteria:t3e:xopj3 [2025/07/04 22:36] – jfpothierbacteria:t3e:xopj3 [2025/07/24 22:33] (current) – jfpothier
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 ====== The Type III Effector XopJ3 from //Xanthomonas// ====== ====== The Type III Effector XopJ3 from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Doron_Teper|Doron Teper]]+Author: [[https://www.researchgate.net/profile/Doron_Teper|Doron Teper]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 AvrRxv/XopJ3 of //X. euvesicatoria// was identified to promote HR in non-host bean plants (Whalen //et al.//, 1988). AvrRxv/XopJ3 of //X. euvesicatoria// was identified to promote HR in non-host bean plants (Whalen //et al.//, 1988).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Tn//5// insertions in multiple hrp genes in //XcvT //75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka //et al.//, 1998). Disruption of the type III secretion system in //Xcv// race T3 abolished secretion of AvrRxv into culture media (Bonshtien //et al.//, 2004).+Tn//5// insertions in multiple hrp genes in //XcvT// 75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka //et al.//, 1998). Disruption of the type III secretion system in //Xcv// race T3 abolished secretion of AvrRxv into culture media (Bonshtien //et al.//, 2004). 
 === Regulation === === Regulation ===
  
-The //avrRxv //gene is constitutively expressed in the //X. euvesicatoria// tomato-pathogenic strain //XcvT// 92-14 (Ciesiolka //et al.//, 1998). The expression of //avrRxv// was similar in NYB and XVM2 medium and was not dependent on //hrpG //or //hrpX // (Ciesiolka //et al.//, 1998). The //avrRxv// gene has a perfect PIP-box starting downstream of the coding sequence at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka //et al.//, 1998).+The //avrRxv// gene is constitutively expressed in the //X. euvesicatoria// tomato-pathogenic strain //XcvT// 92-14 (Ciesiolka //et al.//, 1998). The expression of //avrRxv// was similar in NYB and XVM2 medium and was not dependent on //hrpG//or //hrpX// (Ciesiolka //et al.//, 1998). The //avrRxv// gene has a perfect PIP-box starting downstream of the coding sequence at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka //et al.//, 1998). 
 === Phenotypes === === Phenotypes ===
  
 AvrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen //et al.//, 1993; Bonshtien //et al.//, 2004), bean (Whalen //et al.//, 1988) and //Nicotiana benthamiana// (Albers //et al.//, 2019). Inactivation of //arvRxv// (//avrRxv//::Ω) in //XcvT// 75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al., 1993). //Xcv// race T3 (//X. euvesicatoria// pv. //perforans//) introduced with //avrRxv// induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth in planta and significant alteration in host transcriptional profile (Bonshtien //et al.//, 2004). AvrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen //et al.//, 1993; Bonshtien //et al.//, 2004), bean (Whalen //et al.//, 1988) and //Nicotiana benthamiana// (Albers //et al.//, 2019). Inactivation of //arvRxv// (//avrRxv//::Ω) in //XcvT// 75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al., 1993). //Xcv// race T3 (//X. euvesicatoria// pv. //perforans//) introduced with //avrRxv// induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth in planta and significant alteration in host transcriptional profile (Bonshtien //et al.//, 2004).
 +
 === Localization === === Localization ===
  
 AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien //et al.//, 2004). AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien //et al.//, 2004).
 +
 === Enzymatic function === === Enzymatic function ===
  
 AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma & Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien //et al.//, 2004). AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma & Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien //et al.//, 2004).
 +
 === Interaction partners === === Interaction partners ===
  
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 YES (//X. euvesicatoria//, //X. hortorum//, //X. nasturtii//, //X. axonopodis//) YES (//X. euvesicatoria//, //X. hortorum//, //X. nasturtii//, //X. axonopodis//)
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xopj3.1751664993.txt.gz · Last modified: 2025/07/04 22:36 by jfpothier

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