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--- bacteria:t3e:xopj3 [2023/01/09 10:20] – external edit 127.0.0.1
+++ bacteria:t3e:xopj3 [2025/02/21 13:19] (current) – [Biological function] rkoebnik
@@ Line 1: / Line 1: @@
-====== XopJ3 ======
+====== The Type III Effector XopJ3 from //Xanthomonas// ======
-Author: [[https://www.researchgate.net/profile/Doron_Teper|Doron Teper]]\\
+Author: [[https://www.researchgate.net/profile/Doron_Teper|Doron Teper]]
-Internal reviewer:
 Class: XopJ\\
 Family: XopJ3\\
-Prototype: AvrRxv/XCV0471 (//Xanthomonas euvesicatoria// 85-10)\\
+Prototype: AvrRxv (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 75-3)\\
-RefSeq ID: [[http://www.ncbi.nlm.nih.gov/protein/CAJ22102|CAJ22102]]\\
+GenBank ID: [[http://www.ncbi.nlm.nih.gov/protein/AAA27595.1|AAA27595.1]] (373 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011346137.1|WP_011346137.1]] (373 aa)\\
 D structure: Unknown
@@ Line 14: / Line 14: @@
 === How discovered? ===
-AvrRxv/XopJ3 of //X. euvesicatoria //was identified to promote HR in non-host bean plants (Whalen et al. 1988).
+AvrRxv/XopJ3 of //X. euvesicatoria// was identified to promote HR in non-host bean plants (Whalen //et al.//, 1988).
 === (Experimental) evidence for being a T3E ===
-Tn5 insertions in multiple hrp genes in //XcvT //75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka et al. 1998). Disruption of the type III secretion system in //Xcv //race T3 abolished secretion of AvrRxv into culture media (Bonshtien et al. 2004).
+Tn//5// insertions in multiple hrp genes in //XcvT //75-3 eliminated AvrRxv/XopJ3-mediated HR in tomato line Hawaii 7998 (Ciesiolka //et al.//, 1998). Disruption of the type III secretion system in //Xcv// race T3 abolished secretion of AvrRxv into culture media (Bonshtien //et al.//, 2004).
 === Regulation ===
-//avrRxv //is constitutively expressed in Xanthomonas tomato strain //XcvT //92-14 (Ciesiolka et al. 1998). The expression of avrRxv was similar in NYB and XVM2 and was not dependent on //hrpG //or //hrpX // (Ciesiolka et al 1998). //avrRxv //has a perfect PIP-box starting at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka et al 1998).
+The //avrRxv //gene is constitutively expressed in the //X. euvesicatoria// tomato-pathogenic strain //XcvT// 92-14 (Ciesiolka //et al.//, 1998). The expression of //avrRxv// was similar in NYB and XVM2 medium and was not dependent on //hrpG //or //hrpX // (Ciesiolka //et al.//, 1998). The //avrRxv// gene has a perfect PIP-box starting downstream of the coding sequence at bp 465 with the sequence TTCGC-N15-TTCGC (Ciesiolka //et al.//, 1998).
 === Phenotypes ===
-AvrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen et al. 1993)( Bonshtien et al. 2004), bean (Whalen et al. 1988) and //Nicotiana benthamiana// (Albers et al. 2019). Inactivation of //arvRxv // (//avrRxv//:: Ω ) in //XcvT //75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al. 1993). //Xcv //race T3 (//X. perforans//) introduced with //avrRxv //induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth in planta and significant alteration in host transcriptional profile (Bonshtien et al. 2004).
+AvrRxv induce hypersensitive response in tomato line Hawaii 7998 (Whalen //et al.//, 1993; Bonshtien //et al.//, 2004), bean (Whalen //et al.//, 1988) and //Nicotiana benthamiana// (Albers //et al.//, 2019). Inactivation of //arvRxv// (//avrRxv//::Ω) in //XcvT// 75-3 eliminated HR induction and promoted growth in tomato line Hawaii 7998 (Whalen et al., 1993). //Xcv// race T3 (//X. euvesicatoria// pv. //perforans//) introduced with //avrRxv// induced resistance response in tomato line Hawaii 7998 which was characterized by HR, reduced bacterial growth in planta and significant alteration in host transcriptional profile (Bonshtien //et al.//, 2004).
 === Localization ===
-AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien et al. 2004).
+AvrRxv is localized predominantly to the cytoplasm and is possibly associated with the plasma and nuclear membranes (Bonshtien //et al.//, 2004).
 === Enzymatic function ===
-AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma and Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien et al. 2004).
+AvrRxv/XopJ3 belongs to the group of YopJ-family effectors. YopJ-family effectors share a conserved catalytic triad that is identical to that of the C55 family of cysteine proteases (Ma & Ma, 2016). Mutations in the AvrRxv protease catalytic core impaired elicitation of HR and inhibition of bacterial growth in tomato line Hawaii 7998 (Bonshtien //et al.//, 2004).
 === Interaction partners ===
-AvrRxv interacts with tomato 14-3-3 protein (Whalen et al. 2008).
+AvrRxv interacts with tomato 14-3-3 protein (Whalen //et al.//, 2008).
-<font 18.0pt/inherit;;inherit;;inherit>**Conservation**</font>
+===== Conservation =====
 === In xanthomonads ===
-YES (//X. euvesicatoria//, //X. hortorum// , //X. nasturtii//, //X. axonopodis//)
+YES (//X. euvesicatoria//, //X. hortorum//, //X. nasturtii//, //X. axonopodis//)
 === In other plant pathogens/symbionts ===
-YES (//Pseudomonas syringae// spp. (HopZ-family), //Ralstonia solanacearum// (PopP1), //Acidovorax citrulli//, //Bradyrhizobium //sp., //Mesorhizobium //sp., //Sinorhizobium //sp.)
+YES (//Pseudomonas syringae// spp. (HopZ-family), //Ralstonia solanacearum// (PopP1), //Acidovorax citrulli//, //Bradyrhizobium// sp., //Mesorhizobium// sp., //Sinorhizobium// sp.)
 ===== References =====
+Albers P, Üstün S, Witzel K, Kraner M, Börnke F (2019). A remorin from //Nicotiana benthamiana// interacts with the //Pseudomonas// type-III effector protein HopZ1a and is phosphorylated by the immune-related kinase PBS1. Mol. Plant Microbe Interact. 32: 1229-1242. DOI: [[https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-19-0105-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub  0pubmed|10.1094/MPMI-04-19-0105-R]]
 Bonshtien A, Lev A, Gibly A, Debbie P, Avni A, Sessa G (2005). Molecular properties of the //Xanthomonas// AvrRxv effector and global transcriptional changes determined by its expression in resistant tomato plants. Mol. Plant Microbe Interact. 18: 300-310. DOI: [[https://doi.org/10.1094/MPMI-18-0300|10.1094/MPMI-18-0300]]
 Ciesiolka LD, Hwin T, Gearlds JD, Minsavage GV, Saenz R, Bravo M, Handley V, Conover SM, Zhang H, Caporgno J, Phengrasamy NB, Toms AO, Stall RE, Whalen MC (1999). Regulation of expression of avirulence gene //avrRxv// and identification of a family of host interaction factors by sequence analysis of //avrBsT//. Mol. Plant Microbe Interact. 12: 35-44. DOI: [[https://doi.org/10.1094/MPMI.1999.12.1.35|10.1094/MPMI.1999.12.1.35]]
+Ma KW, Ma W (2016). YopJ family effectors promote bacterial infection through a unique acetyltransferase activity. Microbiol. Mol. Biol. Rev. 80: 1011-1027. DOI: [[https://mmbr.asm.org/content/80/4/1011.long|10.1128/MMBR.00032-16]]
 Whalen MC, Stall RE, Staskawicz BJ (1988). Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. Proc. Natl. Acad. Sci. USA 85: 6743-6747. DOI: [[https://doi.org/10.1073/pnas.85.18.6743|10.1073/pnas.85.18.6743]]
-Whalen MC, Wang JF, Carland FM, Heiskell ME, Dahlbeck D, Minsavage GV, Jones JB, Scott JW, Stall RE, Staskawicz BJ (1993). Avirulence gene //avrRxv// from //Xanthomonas campestris// pv. //vesicatoria// specifies resistance on tomato line Hawaii 7998. Mol. Plant Microbe Interact. 6: 616-627. DOI: [[https://doi.org/10.1094/mpmi-6-616|10.1094/mpmi-6-616]]
+Whalen M, Richter T, Zakhareyvich K, Yoshikawa M, Al-Azzeh D, Adefioye A, Spicer G, Mendoza LL, Morales CQ, Klassen V, Perez-Baron G, Toebe CS, Tzovolous A, Gerstman E, Evans E, Thompson C, Lopez M, Ronald PC (2008). Identification of a host 14-3-3 protein that Interacts with //Xanthomonas// effector AvrRxv. Physiol. Mol. Plant Pathol. 72: 46-55. DOI: [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142867/|10.1016/j.pmpp.2008.05.006]]
-Albers P, Üstün S, Witzel K, Kraner M, Börnke F (2019). A Remorin from //Nicotiana benthamiana// Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1. Mol Plant Microbe Interact. 32:1229-1242. DOI: [[https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-19-0105-R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub  0pubmed|10.1094/MPMI-04-19-0105-R]]
+Whalen MC, Wang JF, Carland FM, Heiskell ME, Dahlbeck D, Minsavage GV, Jones JB, Scott JW, Stall RE, Staskawicz BJ (1993). Avirulence gene //avrRxv// from //Xanthomonas campestris// pv. //vesicatoria// specifies resistance on tomato line Hawaii 7998. Mol. Plant Microbe Interact. 6: 616-627. DOI: [[https://doi.org/10.1094/mpmi-6-616|10.1094/mpmi-6-616]]
-Ma KW, Ma W (2016). YopJ Family Effectors Promote Bacterial Infection through a Unique Acetyltransferase Activity. Microbiol Mol Biol Rev. 80:1011-1027. DOI: [[https://mmbr.asm.org/content/80/4/1011.long|10.1128/MMBR.00032-16]]
+===== Acknowledgements =====
-Whalen M, Richter T, Zakhareyvich K, Yoshikawa M, Al-Azzeh D, Adefioye A, Spicer G, Mendoza LL, Morales CQ, Klassen V, Perez-Baron G, Toebe CS, Tzovolous A, Gerstman E, Evans E, Thompson C, Lopez M, Ronald PC (2008). Identification of a host 14-3-3 Protein that Interacts with //Xanthomonas //effector AvrRxv. Physiol Mol Plant Pathol.72:46-55. DOI: [[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142867/|10.1016/j.pmpp.2008.05.006]]
+This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).

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