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bacteria:t3e:xopj2 [2025/02/13 11:40] – jfpothierbacteria:t3e:xopj2 [2025/07/04 23:37] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Daiva_Burokiene|Daiva Burokienė]]\\ Author: [[https://www.researchgate.net/profile/Daiva_Burokiene|Daiva Burokienė]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Eran_Bosis|Eran Bosis]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Eran_Bosis|Eran Bosis]]\\
-Expert reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Expert reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopJ\\ Class: XopJ\\
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 The //avrBsT// avirulence gene was first identified in the XcvT race 1 strain 75-3, which is avirulent on the pepper cultivar ECW (Minsavage //et al//., 1990). The //avrBsT// gene was localized on an indigenous plasmid of approximately 41 kb in the XcvT race 1 strain 75-3 (Minsavage //et al//., 1990). Spontaneous loss of the plasmid-borne //avrBsT// allowed XcvT race 1 75-3 mutants to cause disease on normally resistant pepper lines, suggesting simple genetic control of nonhost, //avrBsT//-specific resistance (Minsavage //et al//., 1990). The //avrBsT// gene sequence was published in 1999 (Ciesiolka //et al//., 1999). The //avrBsT// avirulence gene was first identified in the XcvT race 1 strain 75-3, which is avirulent on the pepper cultivar ECW (Minsavage //et al//., 1990). The //avrBsT// gene was localized on an indigenous plasmid of approximately 41 kb in the XcvT race 1 strain 75-3 (Minsavage //et al//., 1990). Spontaneous loss of the plasmid-borne //avrBsT// allowed XcvT race 1 75-3 mutants to cause disease on normally resistant pepper lines, suggesting simple genetic control of nonhost, //avrBsT//-specific resistance (Minsavage //et al//., 1990). The //avrBsT// gene sequence was published in 1999 (Ciesiolka //et al//., 1999).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 C-myc epitope-tagged AvrBsT protein was detected in culture supernatants of the //X. euvesicatoria// pv. //euvesicatoria// (//Xee//; ex. //X. campestris// pv. //vesicatoria//) strain 85* only in the presence of a functional type III apparatus and not in a //hrcV// mutant, showing that the protein is secreted in an hrp-dependent manner (Escolar //et al//., 2001). Transient expression of //avrBsT// in resistant host plants using //Agrobacterium tumefaciens//-mediated gene transfer resulted in the induction of a specific HR. This indicates that recognition occurs intracellularly, and suggested that during the //Xee// infection, AvrBsT is translocated from //Xee// into the plant cell (Escolar //et al//., 2001). Mutation studies of a putative translocation motif (TrM) showed that the proline/arginine-rich motif contributes to efficient type III-dependent secretion and translocation of AvrBsT and affects the dependence of AvrBsT transport on the general T3S chaperone HpaB (Prochaska //et al//., 2018). C-myc epitope-tagged AvrBsT protein was detected in culture supernatants of the //X. euvesicatoria// pv. //euvesicatoria// (//Xee//; ex. //X. campestris// pv. //vesicatoria//) strain 85* only in the presence of a functional type III apparatus and not in a //hrcV// mutant, showing that the protein is secreted in an hrp-dependent manner (Escolar //et al//., 2001). Transient expression of //avrBsT// in resistant host plants using //Agrobacterium tumefaciens//-mediated gene transfer resulted in the induction of a specific HR. This indicates that recognition occurs intracellularly, and suggested that during the //Xee// infection, AvrBsT is translocated from //Xee// into the plant cell (Escolar //et al//., 2001). Mutation studies of a putative translocation motif (TrM) showed that the proline/arginine-rich motif contributes to efficient type III-dependent secretion and translocation of AvrBsT and affects the dependence of AvrBsT transport on the general T3S chaperone HpaB (Prochaska //et al//., 2018).
 +
 === Regulation === === Regulation ===
  
 Expression of the //avrBsT// gene is constitutive and independent of the //hrp// gene regulators in //Xee// strain 85-10, //hrpG// and //hrpX// (Escolar //et al//., 2001). Expression of the //avrBsT// gene is constitutive and independent of the //hrp// gene regulators in //Xee// strain 85-10, //hrpG// and //hrpX// (Escolar //et al//., 2001).
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 === Phenotypes === === Phenotypes ===
  
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 Yes (//X. euvesicatoria//, //X. arboricola//, //X. citri//, //X. guizotiae//, //X. phaseoli//, //X. vasicola//, //X. vesicatoria//) (Sharma //et al.//, 2024). However, reports that XopJ2 is conserved in //X. campestris// should be treated with caution, as genome-wide ANI indicates that neither //X. campestris// pv. //parthenii// nor //X. campestris// pv. //coriandri// belong to the species //X. campestris// (Harrison //et al.//, 2023a, 2023b). Yes (//X. euvesicatoria//, //X. arboricola//, //X. citri//, //X. guizotiae//, //X. phaseoli//, //X. vasicola//, //X. vesicatoria//) (Sharma //et al.//, 2024). However, reports that XopJ2 is conserved in //X. campestris// should be treated with caution, as genome-wide ANI indicates that neither //X. campestris// pv. //parthenii// nor //X. campestris// pv. //coriandri// belong to the species //X. campestris// (Harrison //et al.//, 2023a, 2023b).
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 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xopj2.1739446859.txt.gz · Last modified: 2025/02/13 11:40 by jfpothier

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