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bacteria:t3e:xoph [2025/02/24 15:38] – [Further reading] rkoebnikbacteria:t3e:xoph [2025/07/04 23:34] (current) – jfpothier
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 ====== The Type III Effector XopH from //Xanthomonas// ====== ====== The Type III Effector XopH from //Xanthomonas// ======
  
-Author: [[https://orcid.org/0000-0002-4827-2115|Isabel Rodrigues ]]\\ +Author: [[https://www.researchgate.net/profile/Isabel-Rodrigues-12|Isabel Rodrigues]]\\ 
-Internal reviewer: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Fernandes]]+Internal reviewer: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Fernandes]]\\
  
 Class: XopH\\ Class: XopH\\
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 The XopH effector, also known as AvrBs1.1 (White //et al//., 2009), was first reported in 1988 (Ronald and Staskawicz 1988) and discovered due to its virulent activity (Gurenn //et al//., 2006). Later, this effector began to be identified based on the coregulation with the TTS system (Gurlebeck //et al//., 2006), most recently began to be identified by a combination of biochemical approaches, including a new NMR-based method to discriminate inositol polyphosphate enantiomers (Blüher //et al//., 2017). The XopH effector, also known as AvrBs1.1 (White //et al//., 2009), was first reported in 1988 (Ronald and Staskawicz 1988) and discovered due to its virulent activity (Gurenn //et al//., 2006). Later, this effector began to be identified based on the coregulation with the TTS system (Gurlebeck //et al//., 2006), most recently began to be identified by a combination of biochemical approaches, including a new NMR-based method to discriminate inositol polyphosphate enantiomers (Blüher //et al//., 2017).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 The effector XopH, inhibited flg22-induced callose deposition //in planta// (Popov //et al//., 2016), dephosphorylates myo- inositol-hexakisphosphate (phytate, InsP6) to produce InsP5[1-OH], both //in vitro// and //in vivo,// and enhanced disease symptoms (Blüher //et al//., 2017; White and Jones 2018). The xopH activity can led to diminishing amounts of inositol pyrophosphates InsP7 and InsP8 (White and Jones 2018). It was also identified host changes in gene expression due to XopH activity (White and Jones 2018). The effector XopH, inhibited flg22-induced callose deposition //in planta// (Popov //et al//., 2016), dephosphorylates myo- inositol-hexakisphosphate (phytate, InsP6) to produce InsP5[1-OH], both //in vitro// and //in vivo,// and enhanced disease symptoms (Blüher //et al//., 2017; White and Jones 2018). The xopH activity can led to diminishing amounts of inositol pyrophosphates InsP7 and InsP8 (White and Jones 2018). It was also identified host changes in gene expression due to XopH activity (White and Jones 2018).
 +
 === Regulation === === Regulation ===
  
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 This effector can inhibit flg22- but not ABA-inducible reporter gene activation in protoplasts act as PTI inhibitors in planta and contribute to development of disease symptoms like chlorosis (Popov //et al//., 2016). XopH liberates phosphate from the plant tissue to improve the nutritional status of the pathogen what causes the plant show obvious symptoms of phosphorus deficiency (Blüher //et al//., 2017). Transgenic //Nicotiana benthamiana// plants constitutively expressing XopH were significantly smaller than transgenic GFP control plants of the same age and showed signs of early senescence indicating that the effector XopH might affect the ET pathway (Blüher //et al//., 2017). XopH sequester InsP6 by degrading it to an InsP5 isomer, which is not easily metabolized by the plant and accumulates what can compromise plant defense mechanism (Blüher //et al//., 2017). This effector can inhibit flg22- but not ABA-inducible reporter gene activation in protoplasts act as PTI inhibitors in planta and contribute to development of disease symptoms like chlorosis (Popov //et al//., 2016). XopH liberates phosphate from the plant tissue to improve the nutritional status of the pathogen what causes the plant show obvious symptoms of phosphorus deficiency (Blüher //et al//., 2017). Transgenic //Nicotiana benthamiana// plants constitutively expressing XopH were significantly smaller than transgenic GFP control plants of the same age and showed signs of early senescence indicating that the effector XopH might affect the ET pathway (Blüher //et al//., 2017). XopH sequester InsP6 by degrading it to an InsP5 isomer, which is not easily metabolized by the plant and accumulates what can compromise plant defense mechanism (Blüher //et al//., 2017).
 +
 === Localization === === Localization ===
  
 The effector XopH is localized in the nucleus and in the cytoplasm of the plant cell (Popov //et al//., 2016; Blüher //et al//., 2017). The effector XopH is localized in the nucleus and in the cytoplasm of the plant cell (Popov //et al//., 2016; Blüher //et al//., 2017).
 +
 === Enzymatic function === === Enzymatic function ===
  
 XopH is a T3E with phytate-degrading activity, //in vitro// and //in planta// (Blüher //et al//., 2017). XopH is a T3E with phytate-degrading activity, //in vitro// and //in planta// (Blüher //et al//., 2017).
 +
 === Interaction partners === === Interaction partners ===
  
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 Yes (//e.g. Xanthomonas campestris pv. campestris// (Potnis //et al//., 2012), //Xanthomonas arboricola// pv. //corylina// (Hajri //et al//., 2012), //Xanthomonas euvesicatoria// (White and Jones 2018)). Yes (//e.g. Xanthomonas campestris pv. campestris// (Potnis //et al//., 2012), //Xanthomonas arboricola// pv. //corylina// (Hajri //et al//., 2012), //Xanthomonas euvesicatoria// (White and Jones 2018)).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
bacteria/t3e/xoph.1740411539.txt.gz · Last modified: 2025/02/24 15:38 by rkoebnik

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