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bacteria:t3e:xopg [2024/08/06 14:56] – [The Type III Effector XopG from Xanthomonas] rkoebnikbacteria:t3e:xopg [2025/07/04 23:34] (current) – jfpothier
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 ====== The Type III Effector XopG from //Xanthomonas// ====== ====== The Type III Effector XopG from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Ferdandes]]\\+Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Fernandes]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\
-Expert reviewer: [[https://scholar.google.com/citations?user=YqEpuD0AAAAJ&hl=en|Neha Potnis]]+Expert reviewer: [[https://scholar.google.com/citations?user=YqEpuD0AAAAJ&hl=en|Neha Potnis]]\\
  
 Class: XopG\\ Class: XopG\\
-Family: XopG1, XopG2, XopG3\\+Families: XopG1, XopG2, XopG3\\
 Prototype: XCV1298 (XopG1) (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\ Prototype: XCV1298 (XopG1) (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\
 GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAJ22929.1|CAJ22929.1]] (213 aa)\\ GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAJ22929.1|CAJ22929.1]] (213 aa)\\
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 === How discovered? === === How discovered? ===
  
-XopG was identified based on homology searches using TBLASTN analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5 </sup>  (Potnis //et al//., 2011).+XopG was identified based on homology searches using TBLASTN analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5</sup>  (Potnis //et al//., 2011). 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 The T3SS-dependent translocation evidence for XopG protein was confirmed using the AvrBs2 reporter gene assay (Thieme, 2006; Potnis //et al//., 2011). The T3SS-dependent translocation evidence for XopG protein was confirmed using the AvrBs2 reporter gene assay (Thieme, 2006; Potnis //et al//., 2011).
 +
 === Regulation === === Regulation ===
  
 XopG belongs to translocation class B and is still translocated in the absence of HpaB, being constitutively expressed (Schulze et al. 2012). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (da Silva //et al//., 2002; White //et al//., 2009). XopG belongs to translocation class B and is still translocated in the absence of HpaB, being constitutively expressed (Schulze et al. 2012). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (da Silva //et al//., 2002; White //et al//., 2009).
 +
 === Phenotypes === === Phenotypes ===
  
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 //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020). //Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
 +
 === Localization === === Localization ===
  
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 Yes (//e.g.//, //X. arboricola//, //X. campestris//, //X. citri//, //X.// //euvesicatoria//, //X. gardneri//, //X. oryzae////, // //X. translucens//, //X. vesicatoria//). Yes (//e.g.//, //X. arboricola//, //X. campestris//, //X. citri//, //X.// //euvesicatoria//, //X. gardneri//, //X. oryzae////, // //X. translucens//, //X. vesicatoria//).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
 Yes (//Ralstonia solanacearum//, //Pseudomonas// spp., //Acidovorax citrulli//). Yes (//Ralstonia solanacearum//, //Pseudomonas// spp., //Acidovorax citrulli//).
 +
 ===== References ===== ===== References =====
  
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 White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol.10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]] White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol.10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopg.1722952603.txt.gz · Last modified: 2024/08/06 14:56 by rkoebnik

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