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bacteria:t3e:xopg [2020/06/07 20:32] – leonor_martinsbacteria:t3e:xopg [2025/02/18 22:41] (current) – jfpothier
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-====== XopG ======+====== The Type III Effector XopG from //Xanthomonas// ======
  
-Author: Camila Fernandes\\ +Author: [[https://www.researchgate.net/profile/Camila_Fernandes2|Camila Fernandes]]\\ 
-Internal reviewer: Leonor Martins\\ +Internal reviewer: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\ 
-Expert reviewer: Neha Potnis+Expert reviewer: [[https://scholar.google.com/citations?user=YqEpuD0AAAAJ&hl=en|Neha Potnis]]\\
  
 Class: XopG\\ Class: XopG\\
-Family: XopG1, XopG2, XopG3\\ +Families: XopG1, XopG2, XopG3\\ 
-Prototype: XopG (//Xanthomonas euvesicatoria// aka //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\ +Prototype: XCV1298 (XopG1) (//Xanthomonas euvesicatoria// pv. //euvesicatoria//, ex //Xanthomonas campestris// pv. //vesicatoria//; strain 85-10)\\ 
-RefSeq ID: [[http://ensemblgenomes.org/id/CAJ22929|CAJ22929]] (213 aa)\\ +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAJ22929.1|CAJ22929.1]] (213 aa)\\ 
-3D structure: [[https://swissmodel.expasy.org/repository/uniprot/Q3BW34|Q3BW34]] (Q3BW34_XANC5)+RefSeq ID: XopG1 [[https://www.ncbi.nlm.nih.gov/protein/WP_011346758.1|WP_011346758.1]] (213 aa), XopG2 [[https://www.ncbi.nlm.nih.gov/protein/WP_011038360.1|WP_011038360.1]] (207 aa), XopG3 [[https://www.ncbi.nlm.nih.gov/protein/WP_084217840.1|WP_084217840.1]] (226 aa)\\ 
 +3D structure: [[https://swissmodel.expasy.org/repository/uniprot/Q3BW34|Q3BW34]] (homology model)
  
 ===== Biological function ===== ===== Biological function =====
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 === How discovered? === === How discovered? ===
  
-XopG was identified based on homology searches using tblastn analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10 <sup>-5 </sup>  (Potnis //et al//., 2011).+XopG was identified based on homology searches using TBLASTN analysis. Known T3E proteins from plant and animal pathogens were used as query against all contigs of the draft genomes of //X. vesicatoria//, //X. gardneri// and //X. perforans// with e-value of 10<sup>-5</sup>  (Potnis //et al//., 2011).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 === Regulation === === Regulation ===
  
-XopG belongs to class B and is still translocated in the absence of HpaB, being constitutively expressed (Thieme, 2006; White//et al//., 2009). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (White //et al//., 2009; da Silva //et al//., 2002).+XopG belongs to translocation class B and is still translocated in the absence of HpaB, being constitutively expressed (Schulze et al. 2012). XopG was identified as part of the putative HrpX regulon in //X. campestris// pv. //campestris// ATCC339138 (da Silva //et al//., 2002; White //et al//., 2009).
 === Phenotypes === === Phenotypes ===
  
-A deletion of xopG not display differences in the induction of disease symptoms or hypersensibility response. XopG trigger cell death in different Solanaceae, including //Nicotiana// //tabacum// (White //et al//., 2009). XopG could be an essential pathogenicity factor in pepper (Potnis //et al//., 2011).+A deletion of //xopG// did not display differences in the induction of disease symptoms or hypersensibility response. XopG trigger cell death in different //Solanaceae//, including //Nicotiana// //tabacum// (White //et al//., 2009). XopG could be an essential pathogenicity factor in pepper (Potnis //et al//., 2011). 
 + 
 +//Agrobacterium//-mediated transient expression of both XopQ and XopX in rice cells resulted in induction of rice immune responses, which were not observed when either protein was individually expressed. A screen for //Xanthomonas// effectors which can suppress XopQ-XopX induced rice immune responses, led to the identification of five effectors, namely XopU, XopV, XopP, XopG and AvrBs2, that could individually suppress these immune responses. These results suggest a complex interplay of //Xanthomonas// T3SS effectors in suppression of both pathogen-triggered immunity and effector-triggered immunity to promote virulence on rice (Deb //et al.//, 2020).
 === Localization === === Localization ===
  
-Confocal laser scanning microscopy revealed a localization of XopG::GFP exclusively to the plant cell nucleus (White //et al//., 2009).+Confocal laser scanning microscopy revealed a localization of XopG::GFP exclusively to the plant cell nucleus (Schulze et al. 2012). 
 === Enzymatic function === === Enzymatic function ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes (//Xanthomonas// spp.,//X.// //euvesicatoria//, //X. vesicatoria//, //X. gardneri//, //X. campestris// pv. //campestris//, //Xanthomonas campestris// pv. //phaseoli//, //Xanthomonas translucens// pv. //translucens//, //X. translucens// pv. //graminis//, //X. citri pv. bilvae//, //X.citri pv. fuscans//, //X. oryzae// pv. //oryzae//, //X. arboricola// pv. //corylina//, //X. arboricola// pv. //pruni//).+Yes (//e.g.//, //X. arboricola//, //X. campestris//, //X. citri//, //X.// //euvesicatoria//, //X. gardneri//, //X. oryzae////, // //X. translucens//, //X. vesicatoria//).
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 ===== References ===== ===== References =====
  
-da Silva ACR, Ferro JA, Reinach FC, 1Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, et al (2002). Comparison of the genomes of two //Xanthomonas// pathogens with differing host specificities. Nature. 417 (23) : 459-463. DOI: [[https://doi.org/10.1038/417459a|10.1038/417459a]].+da Silva AC, Ferro JA, Reinach FC, Farah CS, Furlan LR, Quaggio RB, Monteiro-Vitorello CB, Van Sluys MA, Almeida NF, Alves LM, do Amaral AM, Bertolini MC, Camargo LE, Camarotte G, Cannavan F, Cardozo J, Chambergo F, Ciapina LP, Cicarelli RM, Coutinho LL, Cursino-Santos JR, El-Dorry H, Faria JB, Ferreira AJ, Ferreira RC, Ferro MI, Formighieri EF, Franco MC, Greggio CC, Gruber A, Katsuyama AM, Kishi LT, Leite RP, Lemos EG, Lemos MV, Locali EC, Machado MA, Madeira AM, Martinez-Rossi NM, Martins EC, Meidanis J, Menck CF, Miyaki CY, Moon DH, Moreira LM, Novo MT, Okura VK, Oliveira MC, Oliveira VR, Pereira HA, Rossi A, Sena JA, Silva C, de Souza RF, Spinola LA, Takita MA, Tamura RE, Teixeira EC, Tezza RI, Trindade dos Santos M, Truffi D, Tsai SM, White FF, Setubal JC, Kitajima JP (2002). Comparison of the genomes of two //Xanthomonas// pathogens with differing host specificities. Nature 417: 459-463. DOI: [[https://doi.org/10.1038/417459a|10.1038/417459a]] 
 + 
 +Deb S, Ghosh P, Patel HK, Sonti RV (2020). Interaction of the //Xanthomonas// effectors XopQ and XopX results in induction of rice immune responses. Plant J. 104: 332-350. DOI: [[https://doi.org/10.1111/tpj.14924|10.1111/tpj.14924]] 
 + 
 +Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol MT, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics 12: 146. DOI: [[https://doi.org/10.1186/1471-2164-12-146|10.1186/1471-2164-12-146]] 
 + 
 +Schulze S, Kay S, Büttner D, Egler M, Eschen-Lippold L, Hause G, Krüger A, Lee J, Müller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from //Xanthomonas// uncovers XopB and XopS as suppressors of plant immunity. New Phytol. 195: 894-911. DOI: [[https://doi.org/10.1111/j.1469-8137.2012.04210.x|10.1111/j.1469-8137.2012.04210.x]]
  
-Potnis N, Krasileva K, Chow V, Almeida NF, Patil PB, Ryan RP, Sharlach M, Behlau F, Dow JM, Momol MT, White FF, Preston JF, Vinatzer BA, Koebnik R, Setubal JC, Norman DJ, Staskawicz BJ, Jones JB (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics. 12 (146) : 1-23. DOI: [[https://doi.org/10.1186/1471-2164-12-146|10.1186/1471-2164-12-146]]+Thieme F (2006). Genombasierte Identifizierung neuer potentieller Virulenzfaktoren von //Xanthomonas// //campestris// pv. //vesicatoria//. Doctoral Thesis, Martin-Luther-Universität Halle-Wittenberg, Germany. PDF: [[http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf|http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf]]
  
-Schulze S, Kay S, Buttner D, Egler M, Eschen-Lippold L, Hause G, Kruger A, Lee J, Muller O, Scheel D, Szczesny R, Thieme F, Bonas U (2012). Analysis of new type III effectors from //Xanthomonas// uncovers XopB and XopS as suppressors of plant immunity. New Phytologist. 195 : 894-911. DOI: [[https://doi.org/10.1111/j.1469-8137.2012.04210.x|10.1111/j.1469-8137.2012.04210.x]].+Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter FJ, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium //Xanthomonas campestris// pv. //vesicatoria// revealed by the complete genome sequence. J. Bacteriol. 187: 7254-7266. DOI: [[https://doi.org/10.1128/JB.187.21.7254-7266.2005|10.1128/JB.187.21.7254-7266.2005]]
  
-Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Büttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klösgen U, Patschkowski T, Rückert C, Rupp O, Schneiker S, Schuster SC, Vorhölter FJ, Weber E, Pühler A, Bonas U, Bartels D, Kaiser O (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium //Xanthomonas campestris// pv. //vesicatoria// revealed by the complete genome sequence. J Bacteriol. 187 (21) : 7254-7266. DOI: [[https://doi.org/10.1128/JB.187.21.7254-7266.2005|10.1128/JB.187.21.7254-7266.2005]].+White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol.10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]]
  
-Thieme F (2006). Genombasierte Identifizierung neuer potentieller Virulenzfaktoren von //Xanthomonas// //campestris// pv. //vesicatoria//. PhD dissertation. [[http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf|http://sundoc.bibliothek.uni-halle.de/diss-online/06/06H103/prom.pdf]]+===== Acknowledgements =====
  
-White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Molecular Plant Pathology.10 (6) : 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]].+This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopg.1591558375.txt.gz · Last modified: 2023/01/09 10:20 (external edit)

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