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bacteria:t3e:xopf [2025/02/13 11:31] – jfpothierbacteria:t3e:xopf [2025/07/24 22:31] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\ Author: [[https://www.researchgate.net/profile/Leonor_Martins|Leonor Martins]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\ Internal reviewer: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\
-Expert reviewer: [[https://www.researchgate.net/profile/Kalyan_Mondal|Kalyan K Mondal]]+Expert reviewer: [[https://www.researchgate.net/profile/Kalyan_Mondal|Kalyan K Mondal]]\\
  
 Class: XopF\\ Class: XopF\\
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 XopF1 and XopF2 were identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopF1::AvrBs2 and XopF2::AvrBs2 fusion proteins triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004). XopF1 and XopF2 were identified in a genetic screen, using a Tn//5//-based transposon construct harboring the coding sequence for the HR-inducing domain of AvrBs2, but devoid of the effectors' T3SS signal, that was randomly inserted into the genome of //X. campestris// pv. //vesicatoria// (//Xcv//) strain 85-10. The XopF1::AvrBs2 and XopF2::AvrBs2 fusion proteins triggered a //Bs2//-dependent hypersensitive response (HR) in pepper leaves (Roden //et al//., 2004).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an //Xcv// chaperone (Roden //et al//., 2004). XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an //Xcv// chaperone (Roden //et al//., 2004).
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 === Regulation === === Regulation ===
  
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 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016).
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 === Phenotypes === === Phenotypes ===
  
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   * Additionally, XopF1 and XopF2 of //X. euvesicatoria// and //Xoo// seem to have a role in PTI suppression //in planta//, namely by inhibiting callose deposition and by suppressing the induction of PTI marker genes, overall contributing to development of symptoms (Mondal //et al//., 2016; Popov //et al//., 2016).   * Additionally, XopF1 and XopF2 of //X. euvesicatoria// and //Xoo// seem to have a role in PTI suppression //in planta//, namely by inhibiting callose deposition and by suppressing the induction of PTI marker genes, overall contributing to development of symptoms (Mondal //et al//., 2016; Popov //et al//., 2016).
   * //Xoo// XopF1 triggered an HR in non-host plants (Li //et al//., 2016).   * //Xoo// XopF1 triggered an HR in non-host plants (Li //et al//., 2016).
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 === Localization === === Localization ===
  
bacteria/t3e/xopf.1739446291.txt.gz · Last modified: 2025/02/13 11:31 by jfpothier

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