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bacteria:t3e:xopf [2020/07/08 16:06] – [Further reading] rkoebnik | bacteria:t3e:xopf [2025/02/13 11:31] (current) – jfpothier | ||
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- | ====== XopF ====== | + | ====== |
Author: [[https:// | Author: [[https:// | ||
Internal reviewer: [[https:// | Internal reviewer: [[https:// | ||
- | Expert reviewer: | + | Expert reviewer: |
Class: XopF\\ | Class: XopF\\ | ||
- | Family: XopF1, XopF2, XopF3\\ | + | Families: XopF1, XopF2, XopF3\\ |
- | Prototype: | + | Prototype: |
- | RefSeq ID: XopF1 [[https:// | + | GenBank ID (XopF1): |
+ | GenBank ID (XopF2): [[https:// | ||
+ | GenBank ID (XopF3): [[https:// | ||
+ | RefSeq ID: XopF1 [[https:// | ||
+ | Synonym: Hpa4\\ | ||
3D structure: Unknown | 3D structure: Unknown | ||
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=== How discovered? === | === How discovered? === | ||
- | XopF1 and XopF2 were identified in a genetic screen, using a Tn// | + | XopF1 and XopF2 were identified in a genetic screen, using a Tn// |
=== (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
- | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004). | + | Type III-dependent secretion of XopF1 and XopF2 was confirmed using a calmodulin-dependent adenylate cyclase reporter assay, with a Δ//hrpF// mutant strain serving as negative control (Roden //et al.//, 2004, Mondal //et al.//, 2016). |
Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | Fragments of the //xopF1// gene are located within the //hrp// cluster of many // | ||
- | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an Xcv chaperone (Roden //et al//., 2004). | + | XopF1 belongs to the class A effectors (Büttner //et al//., 2006). XopF2 is 59% identical and 68% similar to XopF1 when analysed with the pairwise BLAST algorithm. //xopF2// appears to be co-transcribed with ORF1. ORF1 analysis revealed characteristics shared by type III chaperones, and is suggested to encode an //Xcv// chaperone (Roden //et al//., 2004). |
=== Regulation === | === Regulation === | ||
RT-PCR analysis revealed //xopF1// is regulated by //hrpG// and //hrpX// and that //xopF1//, //hpaD//, //hpaI// belong to the same operon. Upstream there is a PIP box which provides binding site for HrpX (Büttner //et al//., 2007). | RT-PCR analysis revealed //xopF1// is regulated by //hrpG// and //hrpX// and that //xopF1//, //hpaD//, //hpaI// belong to the same operon. Upstream there is a PIP box which provides binding site for HrpX (Büttner //et al//., 2007). | ||
- | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the // | + | qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopF//, were significantly reduced in the // |
=== Phenotypes === | === Phenotypes === | ||
- | * Roden et al. did not find significant growth defects of a // | + | * Roden et al. did not find significant growth defects of a //Xcv// Δ//xopF1// or Δ//xopF2// mutant in susceptible pepper and tomato leaves (Roden |
- | * To study the possible virulence function of the putative // | + | * To study the possible virulence function of the putative //xopF1// operon encoding HpaD, HpaI, and XopF1 these three genes were deleted from the genome of //Xcv// 85-10. The resultant mutant strain 85-10Δ// |
- | * Later, // | + | * Later, //Xoo// XopF1 was proven to contribute to virulence in rice, as infection with //xopF1// mutant has shown a reduced lesion size comparing to wild type (Mondal //et al//., 2016). |
- | * Additionally, | + | * Additionally, |
- | * // | + | * //Xoo// XopF1 triggered an HR in non-host plants (Li //et al//., 2016). |
=== Localization === | === Localization === | ||
- | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within // | + | XopF2 localizes in the Golgi apparatus, while XopF1 has been found in cytoplasm (Popov //et al//., 2016) and plasma membrane (Mondal //et al//., 2016). XopF1 is encoded within //hrp// region, between //hpaB// and //hrpF//, while XopF2 is encoded elsewhere in the bacterial chromosome (Roden //et al//., 2004; Büttner //et al//., 2007). |
=== Enzymatic function === | === Enzymatic function === | ||
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=== In xanthomonads === | === In xanthomonads === | ||
- | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae//, //X. euvesicatoria//, | + | Yes (//e.g.//, //X. arboricola, X. bromi//, //X. citri, X. oryzae// pv. //oryzae//, //X. euvesicatoria//, |
=== In other plant pathogens/ | === In other plant pathogens/ | ||
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===== References ===== | ===== References ===== | ||
- | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // | + | Büttner D, Lorenz C, Weber E, Bonas U (2006). Targeting of two effector protein classes to the type III secretion system by a HpaC- and HpaB-dependent protein complex from // |
- | Büttner D, Noël L, Stuttmann J, Bonas U (2007). Characterization of the nonconserved // | + | Büttner D, Noël L, Stuttmann J, Bonas U (2007). Characterization of the nonconserved // |
- | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // | + | Li S, Wang Y, Wang S, Fang A, Wang J, Liu L, Zhang K, Mao Y, Sun W (2015). The type III effector AvrBs2 in // |
- | Liu Y, Long J, Shen D, Song C (2016). // | + | Liu Y, Long J, Shen D, Song C (2016). // |
- | Mondal K K, Verma G, Manju, Junaid A, Mani C (2016). Rice pathogen // | + | Mondal K K, Verma G, Manju, Junaid A, Mani C (2016). Rice pathogen // |
- | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // | + | Popov G, Fraiture M, Brunner F, Sessa G (2016). Multiple // |
- | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | + | Roden J, Belt B, Ross J, Tachibana T, Vargas J, Mudgett M (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // |
===== Further reading ===== | ===== Further reading ===== | ||
- | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of Xanthomonas campestris pv. // | + | Salomon D, Dar D, Sreeramulu S, Sessa G (2011). Expression of //Xanthomonas campestris// pv. // |
+ | |||
+ | ===== Acknowledgements ===== | ||
+ | |||
+ | This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology). | ||