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bacteria:t3e:xope4 [2020/06/30 17:33] – [XopE4] rkoebnikbacteria:t3e:xope4 [2025/07/24 22:28] (current) – jfpothier
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-====== XopE4 ======+====== The Type III Effector XopE4 from //Xanthomonas// ======
  
-Author: Jaime Cubero\\ +Author: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\ 
-Internal reviewer: Eran Bosis\\ +Internal reviewer: [[https://www.researchgate.net/profile/Eran_Bosis|Eran Bosis]]\\ 
-Expert reviewer: FIXME+Expert reviewer: [[https://www.researchgate.net/profile/Adriana_Bernal|Adriana Bernal]]\\
  
 Class: XopE\\ Class: XopE\\
 Family: XopE4\\ Family: XopE4\\
-Prototype: XAUC_31730 (//X. fuscans pv. aurantifolii//)\\ +Prototype: XAUC_31730 (//Xanthomonas fuscans// pv. //aurantifolii//)\\ 
-Protein Accession ID: [[https://www.ncbi.nlm.nih.gov/protein/EFF46466.1|EFF46466.1]] (388 aa)\\ +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/EFF46466.1|EFF46466.1]] (388 aa - likely too long)\\ 
-3D structure: unknown.+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_244170787.1|WP_244170787.1]] (367 aa)\\ 
 +3D structure: unknown
  
 ===== Biological function ===== ===== Biological function =====
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 XopE4 was first identified by sequence homology searches (Moreira //et al//., 2010). XopE4 was first identified by sequence homology searches (Moreira //et al//., 2010).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 The gene sequence of xopE4 is similar to XopE2 (avrXacE3), but due to its low amino acid sequence identity (31%) was considered a different effector that can discriminate between X. citri and //X. fuscans// pv. aurantifolii strains, both causing citrus bacterial canker (Moreira //et al//., 2010; Dalio //et al//., 2017). Disease symptoms caused by //Xanthomonas axonopodis// pv. //manihotis// mutant strains deleted for //xopE4// are similar to those caused by the wild-type strain. Moreover, using heterologous systems XopE4 was unable to suppress (PAMP)-triggered immunity (PTI) but showed weak ability to suppress effector-triggered immunity (ETI) (Medina //et al//., 2018). The gene sequence of xopE4 is similar to XopE2 (avrXacE3), but due to its low amino acid sequence identity (31%) was considered a different effector that can discriminate between X. citri and //X. fuscans// pv. aurantifolii strains, both causing citrus bacterial canker (Moreira //et al//., 2010; Dalio //et al//., 2017). Disease symptoms caused by //Xanthomonas axonopodis// pv. //manihotis// mutant strains deleted for //xopE4// are similar to those caused by the wild-type strain. Moreover, using heterologous systems XopE4 was unable to suppress (PAMP)-triggered immunity (PTI) but showed weak ability to suppress effector-triggered immunity (ETI) (Medina //et al//., 2018).
 +
 === Localization === === Localization ===
  
 As XopE4 does not have a predicted myristoylation site, suggesting that it may not be targeted to the cell membrane as the other XopE family member (Moreira //et al//., 2010). As XopE4 does not have a predicted myristoylation site, suggesting that it may not be targeted to the cell membrane as the other XopE family member (Moreira //et al//., 2010).
 +
 === Enzymatic function === === Enzymatic function ===
  
 XopE4 belongs to the HopX effector family, which are part of the transglutaminase superfamily (Nichmuk //et al//., 2007). XopE4 belongs to the HopX effector family, which are part of the transglutaminase superfamily (Nichmuk //et al//., 2007).
 +
 === Interaction partners === === Interaction partners ===
  
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 Yes (//e.g.//, //X. axonopodis//). Yes (//e.g.//, //X. axonopodis//).
 +
 +XopE4 is also present in //X. fragariae// (Vandroemme //et al//., 2013) and it is not very conserved among //X. perforans// strains (Schwartz //et al//., 2015).
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 Nimchuk ZL, Fisher EJ, Desvaux D, Chang JH, Dangl JL (2007). The HopX (AvrPphE) family of //Pseudomonas syringae// type III effectors require a catalytic triad and a novel N-terminal domain forfunction. Mol. Plant Microbe Interact. 20: 346-357. DOI: [[https://doi.org/10.1094/MPMI-20-4-0346|10.1094/MPMI-20-4-0346]]. Nimchuk ZL, Fisher EJ, Desvaux D, Chang JH, Dangl JL (2007). The HopX (AvrPphE) family of //Pseudomonas syringae// type III effectors require a catalytic triad and a novel N-terminal domain forfunction. Mol. Plant Microbe Interact. 20: 346-357. DOI: [[https://doi.org/10.1094/MPMI-20-4-0346|10.1094/MPMI-20-4-0346]].
 +
 +Schwartz, A. R., Potnis, N., Timilsina, S., Wilson, M., Patané, J., Martins Jr, J., & Vallad, G. E. (2015). Phylogenomics of //Xanthomonas// field strains infecting pepper and tomato reveals diversity in effector repertoires and identifies determinants of host specificity. Front. Microbiol. 6: 535. DOI: [[https://doi.org/10.3389/fmicb.2015.00535|10.3389/fmicb.2015.00535]]
 +
 +Vandroemme, J., Cottyn, B., Baeyen, S., De Vos, P., & Maes, M. (2013). Draft genome sequence of //Xanthomonas fragariae// reveals reductive evolution and distinct virulence-related gene content. BMC Genomics 14: 829. DOI: [[https://doi.org/10.1186/1471-2164-14-829|10.1186/1471-2164-14-829 ]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xope4.1593534811.txt.gz · Last modified: 2023/01/09 10:20 (external edit)

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