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bacteria:t3e:xope3 [2024/08/06 14:54] – [The Type III Effector XopE3 from Xanthomonas] rkoebnikbacteria:t3e:xope3 [2025/02/12 23:55] (current) – jfpothier
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 GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/AAM38068.1|AAM38068.1]] (356 aa)\\ GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/AAM38068.1|AAM38068.1]] (356 aa)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011052114.1|WP_011052114.1]] (356 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011052114.1|WP_011052114.1]] (356 aa)\\
-Synonym: AvrXacE2 (//Xanthomonas citri //pv. //citri//)\\+Synonym: AvrXacE2 (//Xanthomonas citri// pv. //citri//)\\
 3D structure: Contains a catalytic triad of cysteine, histidine and aspartic acid, and have been grouped with peptide N-glycanases (PNGases, members of the transglutaminase protein superfamily). XopE3 contains N-myristoylation motifs (Dunger //et al//., 2012). 3D structure: Contains a catalytic triad of cysteine, histidine and aspartic acid, and have been grouped with peptide N-glycanases (PNGases, members of the transglutaminase protein superfamily). XopE3 contains N-myristoylation motifs (Dunger //et al//., 2012).
- 
 ===== Biological function ===== ===== Biological function =====
  
 === How discovered? === === How discovered? ===
  
-The gene coding for XopE3 (avrXacE2) was first identified in the genome annotation of //Xanthomonas citri //subsp. //citri //A306 (da Silva //et al//., 2002).+The gene coding for XopE3 (avrXacE2) was first identified in the genome annotation of //Xanthomonas citri// subsp. //citri// A306 (da Silva //et al//., 2002).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 === Regulation === === Regulation ===
  
-avrXacE2 was shown to be regulated by HrpG regulon in X. citri (Guo //et al//., 2011). This effector does not contain PIP box-like sequences.+avrXacE2 was shown to be regulated by HrpG regulon in //X. citri// (Guo //et al//., 2011). This effector does not contain PIP box-like sequences.
 === Phenotypes === === Phenotypes ===
  
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 === Interaction partners === === Interaction partners ===
  
-In //X. citri //subsp. //citri //A306 the gene coding for XopE3 is in a region hypothesized to be a genomic island (Moreira //et al.//, 2010). This region or parts of it are conserved in many Xanthomonas strains, as shown by a genomic neighborhood search in the Integrated Microbial Genomes platform. In particular, in this search gene XAC3225 is nearly always adjacent to XAC3224 (//xopE3//), suggesting that the protein coded by XAC3225 is an interaction partner of XopE3. Moreira //et al.// (2010) commented on this as follows: "Next to //xopE3// (XAC3224) we find gene XAC3225, whose product is annotated as tranglycosylase //mltB//. This gene has strong similarity (e-value 10<sup>-133</sup>  , 100% coverage) to //hopAJ1// from //P. syringae// pv. //tomato// strain DC3000, where it is annotated as a T3SS helper protein. Although the //hopAJ1// gene is not itself a T3SS substrate, it contributes to effector translocation (Oh //et al.//, 2007). A mutant with a deletion of XAC3225 has reduced ability to cause canker (mutant phenotypes include a reduction in water soaking, hyperplasia, and necrosis compared to wild type) (Laia //et al.//, 2009)". +In //X. citri// subsp. //citri// A306 the gene coding for XopE3 is in a region hypothesized to be a genomic island (Moreira //et al.//, 2010). This region or parts of it are conserved in many //Xanthomonas// strains, as shown by a genomic neighborhood search in the Integrated Microbial Genomes platform. In particular, in this search gene XAC3225 is nearly always adjacent to XAC3224 (//xopE3//), suggesting that the protein coded by XAC3225 is an interaction partner of XopE3. Moreira //et al.// (2010) commented on this as follows: "Next to //xopE3// (XAC3224) we find gene XAC3225, whose product is annotated as tranglycosylase //mltB//. This gene has strong similarity (e-value 10<sup>-133</sup>, 100% coverage) to //hopAJ1// from //P. syringae// pv. //tomato// strain DC3000, where it is annotated as a T3SS helper protein. Although the //hopAJ1// gene is not itself a T3SS substrate, it contributes to effector translocation (Oh //et al.//, 2007). A mutant with a deletion of XAC3225 has reduced ability to cause canker (mutant phenotypes include a reduction in water soaking, hyperplasia, and necrosis compared to wild type) (Laia //et al.//, 2009)".
 ===== Conservation ===== ===== Conservation =====
  
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 Oh HS, Kvitko BH, Morello JE, Collmer A (2007). //Pseudomonas syringae// lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J. Bacteriol. 189: 8277-8289. DOI: [[https://doi.org/10.1128/JB.00998-07|10.1128/JB.00998-07]] Oh HS, Kvitko BH, Morello JE, Collmer A (2007). //Pseudomonas syringae// lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J. Bacteriol. 189: 8277-8289. DOI: [[https://doi.org/10.1128/JB.00998-07|10.1128/JB.00998-07]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xope3.1722952491.txt.gz · Last modified: 2024/08/06 14:54 by rkoebnik

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