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bacteria:t3e:xope3 [2020/09/21 09:37] – [Biological function] rkoebnikbacteria:t3e:xope3 [2025/02/12 23:55] (current) – jfpothier
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-====== XopE3 ======+====== The Type III Effector XopE3 from //Xanthomonas// ======
  
 Author: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\ Author: [[https://www.researchgate.net/profile/Jaime_Cubero|Jaime Cubero]]\\
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 Class: XopE\\ Class: XopE\\
 Family: XopE3\\ Family: XopE3\\
-Prototype: XAC3224 (//Xanthomonas citri// pv. //citri//)\\+Prototype: XAC3224 (//Xanthomonas citri// pv. //citri//; strain 306)\\ 
 +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/AAM38068.1|AAM38068.1]] (356 aa)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011052114.1|WP_011052114.1]] (356 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011052114.1|WP_011052114.1]] (356 aa)\\
-Synonym: AvrXacE2 (//Xanthomonas citri //pv. //citri//)\\+Synonym: AvrXacE2 (//Xanthomonas citri// pv. //citri//)\\
 3D structure: Contains a catalytic triad of cysteine, histidine and aspartic acid, and have been grouped with peptide N-glycanases (PNGases, members of the transglutaminase protein superfamily). XopE3 contains N-myristoylation motifs (Dunger //et al//., 2012). 3D structure: Contains a catalytic triad of cysteine, histidine and aspartic acid, and have been grouped with peptide N-glycanases (PNGases, members of the transglutaminase protein superfamily). XopE3 contains N-myristoylation motifs (Dunger //et al//., 2012).
 ===== Biological function ===== ===== Biological function =====
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 === How discovered? === === How discovered? ===
  
-The gene coding for XopE3 (avrXacE2) was first identified in the genome annotation of //Xanthomonas citri //subsp. //citri //A306 (da Silva //et al//., 2002).+The gene coding for XopE3 (avrXacE2) was first identified in the genome annotation of //Xanthomonas citri// subsp. //citri// A306 (da Silva //et al//., 2002).
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
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 === Regulation === === Regulation ===
  
-avrXacE2 was shown to be regulated by HrpG regulon in X. citri (Guo //et al//., 2011). This effector does not contain PIP box-like sequences.+avrXacE2 was shown to be regulated by HrpG regulon in //X. citri// (Guo //et al//., 2011). This effector does not contain PIP box-like sequences.
 === Phenotypes === === Phenotypes ===
  
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 === Interaction partners === === Interaction partners ===
  
-In //X. citri //subsp. //citri //A306 the gene coding for XopE3 is in a region hypothesized to be a genomic island (Moreira //et al.//, 2010). This region or parts of it are conserved in many Xanthomonas strains, as shown by a genomic neighborhood search in the Integrated Microbial Genomes platform. In particular, in this search gene XAC3225 is nearly always adjacent to XAC3224 (//xopE3//), suggesting that the protein coded by XAC3225 is an interaction partner of XopE3. Moreira //et al.// (2010) commented on this as follows: "Next to //xopE3// (XAC3224) we find gene XAC3225, whose product is annotated as tranglycosylase //mltB//. This gene has strong similarity (e-value 10<sup>-133</sup>  , 100% coverage) to //hopAJ1// from //P. syringae// pv. //tomato// strain DC3000, where it is annotated as a T3SS helper protein. Although the //hopAJ1// gene is not itself a T3SS substrate, it contributes to effector translocation (Oh //et al.//, 2007). A mutant with a deletion of XAC3225 has reduced ability to cause canker (mutant phenotypes include a reduction in water soaking, hyperplasia, and necrosis compared to wild type) (Laia //et al.//, 2009)". +In //X. citri// subsp. //citri// A306 the gene coding for XopE3 is in a region hypothesized to be a genomic island (Moreira //et al.//, 2010). This region or parts of it are conserved in many //Xanthomonas// strains, as shown by a genomic neighborhood search in the Integrated Microbial Genomes platform. In particular, in this search gene XAC3225 is nearly always adjacent to XAC3224 (//xopE3//), suggesting that the protein coded by XAC3225 is an interaction partner of XopE3. Moreira //et al.// (2010) commented on this as follows: "Next to //xopE3// (XAC3224) we find gene XAC3225, whose product is annotated as tranglycosylase //mltB//. This gene has strong similarity (e-value 10<sup>-133</sup>, 100% coverage) to //hopAJ1// from //P. syringae// pv. //tomato// strain DC3000, where it is annotated as a T3SS helper protein. Although the //hopAJ1// gene is not itself a T3SS substrate, it contributes to effector translocation (Oh //et al.//, 2007). A mutant with a deletion of XAC3225 has reduced ability to cause canker (mutant phenotypes include a reduction in water soaking, hyperplasia, and necrosis compared to wild type) (Laia //et al.//, 2009)".
 ===== Conservation ===== ===== Conservation =====
  
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 Guo Y, Figueiredo F, Jones J, Wang N (2011). HrpG and HrpX play global roles in coordinating different virulence traits of //Xanthomonas axonopodis// pv. citri. Mol Plant Microbe Interact. 24: 649-661. DOI: [[https://doi.org/10.1094/MPMI-09-10-0209|10.1094/MPMI-09-10-0209]] Guo Y, Figueiredo F, Jones J, Wang N (2011). HrpG and HrpX play global roles in coordinating different virulence traits of //Xanthomonas axonopodis// pv. citri. Mol Plant Microbe Interact. 24: 649-661. DOI: [[https://doi.org/10.1094/MPMI-09-10-0209|10.1094/MPMI-09-10-0209]]
  
-Laia ML, Moreira LM, Dezajacomo J, Brigati JB, Ferreira CB, Ferro MI, Silva AC, Ferro JA, Oliveira JC (2009). New genes of //Xanthomonas citri// subsp. //citri// involved in pathogenesis and adaptation revealed by a transposon-based mutant library. BMC Microbiol. 2009, 9: 12. DOI: [[https://doi.org/10.1186/1471-2180-9-12|10.1186/1471-2180-9-12]]+Laia ML, Moreira LM, Dezajacomo J, Brigati JB, Ferreira CB, Ferro MI, Silva AC, Ferro JA, Oliveira JC (2009). New genes of //Xanthomonas citri// subsp. //citri// involved in pathogenesis and adaptation revealed by a transposon-based mutant library. BMC Microbiol. 9: 12. DOI: [[https://doi.org/10.1186/1471-2180-9-12|10.1186/1471-2180-9-12]]
  
 Moreira LM, Almeida NF, Potnis N, Digiampietri LA, Adi SS, Bortolossi JC, da Silva AC, da Silva AM, de Moraes FE, de Oliveira JC, de Souza RF (2010). Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of //Xanthomonas fuscans// subsp. //aurantifolii//. BMC Genomics 11: 238. DOI: [[https://doi.org/10.1186/1471-2164-11-238|10.1186/1471-2164-11-238]] Moreira LM, Almeida NF, Potnis N, Digiampietri LA, Adi SS, Bortolossi JC, da Silva AC, da Silva AM, de Moraes FE, de Oliveira JC, de Souza RF (2010). Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of //Xanthomonas fuscans// subsp. //aurantifolii//. BMC Genomics 11: 238. DOI: [[https://doi.org/10.1186/1471-2164-11-238|10.1186/1471-2164-11-238]]
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 Oh HS, Kvitko BH, Morello JE, Collmer A (2007). //Pseudomonas syringae// lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J. Bacteriol. 189: 8277-8289. DOI: [[https://doi.org/10.1128/JB.00998-07|10.1128/JB.00998-07]] Oh HS, Kvitko BH, Morello JE, Collmer A (2007). //Pseudomonas syringae// lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J. Bacteriol. 189: 8277-8289. DOI: [[https://doi.org/10.1128/JB.00998-07|10.1128/JB.00998-07]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xope3.1600677424.txt.gz · Last modified: 2023/01/09 10:20 (external edit)

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