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bacteria:t3e:xopc

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bacteria:t3e:xopc [2025/02/12 23:50] – jfpothierbacteria:t3e:xopc [2025/12/18 09:52] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Alice_Castaing|Alice Boulanger]]\\ Author: [[https://www.researchgate.net/profile/Alice_Castaing|Alice Boulanger]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopC\\ Class: XopC\\
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 Translocation of the XopC::AvrBs3 chimeric protein was independent of the export control protein, HpaC (Büttner //et al//., 2006). Translocation of the XopC::AvrBs3 chimeric protein was independent of the export control protein, HpaC (Büttner //et al//., 2006).
 +
 === Regulation === === Regulation ===
  
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 qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopC//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016). qRT-PCR revealed that transcript levels of 15 out of 18 tested non-TAL effector genes (as well as the regulatory genes //hrpG// and //hrpX//), including //xopC//, were significantly reduced in the //Xanthomonas oryzae// pv. //oryzae// Δ//xrvC// mutant compared with those in the wild-type strain PXO99<sup>A</sup> (Liu //et al.//, 2016).
 +
 === Phenotypes === === Phenotypes ===
  
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 Yeast-2-hybrid studies revealed a XopC interactor, which also interacted with XopC //in planta//. The interactor localises to the plant cell cytoplasm and carries typical features of plant cytosolic //O//-acetylserine (thiol)lyases (OAS-TL). It shows OAS-TL activity //in vivo// and //in vitro//. The latter one is enhanced by adding XopC (Herzfeld, 2013). Yeast-2-hybrid studies revealed a XopC interactor, which also interacted with XopC //in planta//. The interactor localises to the plant cell cytoplasm and carries typical features of plant cytosolic //O//-acetylserine (thiol)lyases (OAS-TL). It shows OAS-TL activity //in vivo// and //in vitro//. The latter one is enhanced by adding XopC (Herzfeld, 2013).
  
-===== Conservation ===== 
- 
-=== In xanthomonads === 
- 
-Close, full-length homologs (>90% sequence identity) of XopC1 have only been found in several strains of clade-2 xanthomonads, such as //X. citri//, //X. euvesicatoria//, //X. fragariae//, //X. gardneri//, //X. hortorum//, and //X. phaseoli// (BLASTP and TBLASTN performed in June 2020). 
- 
-The distantly related XopC2 has homologs in //X. citri//, //X. axonopodis//, //X. euvesicatoria//, //X. oryzae//, //X. phaseoli//, and //X. translucens// (BLASTP and TBLASTN performed in June 2020) 
- 
-=== In other plant pathogens/symbionts === 
- 
-XopC1: //Ralstonia solanacearum// (RipC2), //Trinickia caryophylli// (//Paraburkholderia caryophylli//), //Xylophilus ampelinus// (BLASTP and TBLASTN performed in June 2020). 
- 
-XopC2: //Acidovorax// ssp., //Pseudomonas cissicola//, //Ralstonia solanacearum// (RipC1) (BLASTP and TBLASTN performed in June 2020). 
  
 ===== Conservation ===== ===== Conservation =====
bacteria/t3e/xopc.1739404238.txt.gz · Last modified: 2025/02/12 23:50 by jfpothier

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